DOI: 10.1128/jb.00844-10. Complete Genome Sequence of <i>Methanothermobacter marburgensis</i> , a Methanoarchaeon Model Organism

Complete Genome Sequence of Methanothermobacter marburgensis , a Methanoarchaeon Model Organism

10.1128/jb.00844-10

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The circular genome sequence of the chemolithoautotrophic euryarchaeon Methanothermobacter marburgensis , with 1,639,135 bp, was determined and compared with that of Methanothermobacter thermautotrophicus . The genomes of the two model methanogens differ substantially in protein coding sequences, in insertion sequence (IS)-like elements, and in clustered regularly interspaced short palindromic repeats (CRISPR) loci.

Bibliography

Liesegang, H., Kaster, A.-K., Wiezer, A., Goenrich, M., Wollherr, A., Seedorf, H., Gottschalk, G., & Thauer, R. K. (2010). Complete Genome Sequence of Methanothermobacter marburgensis , a Methanoarchaeon Model Organism. Journal of Bacteriology, 192(21), 5850â5851.

Authors 8

Heiko Liesegang (first)
Anne-Kristin Kaster (additional)
Arnim Wiezer (additional)
Meike Goenrich (additional)
Antje Wollherr (additional)
Henning Seedorf (additional)
Gerhard Gottschalk (additional)
Rudolf K. Thauer (additional)

References 20 Referenced 31

Bokranz, M., A. Klein, and L. Meile. 1990. Complete nucleotide sequence of plasmid pME2001 of Methanobacterium thermoautotrophicum (Marburg). Nucleic Acids Res. 18 : 363. (10.1093/nar/18.2.363) / Nucleic Acids Res. (1990)
Boone, D. R., W. B. Whitman, and P. Rouvière. 1993. Diversity and taxonomy of methanogens, p. 35-80. In J. G. Ferry (ed.), Methanogenesis. Chapman & Hall, New York, NY. / Methanogenesis. (1993)
Brandis, A., R. K. Thauer, and K. O. Stetter. 1981. Relatedness of strains DH and Marburg of Methanobacterium thermoautotrophicum. Zentralbl. Bakt. Hyg. I Abt. Orig. C 2 : 311-317. / Zentralbl. Bakt. Hyg. I Abt. Orig. C (1981)
Dimarco, A. A., T. A. Bobik, and R. S. Wolfe. 1990. Unusual coenzymes of methanogenesis. Annu. Rev. Biochem. 59 : 355-394. (10.1146/annurev.bi.59.070190.002035) / Annu. Rev. Biochem. (1990)
Fuchs, G., E. Stupperich, and R. K. Thauer. 1978. Acetate assimilation and the synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum. Arch. Microbiol. 117 : 61-66. (10.1007/BF00689352) / Arch. Microbiol. (1978)
Jordan, M., L. Meile, and T. Leisinger. 1989. Organization of Methanobacterium thermoautotrophicum bacteriophage psi M1 DNA. Mol. Gen. Genet. 220 : 161-164. (10.1007/BF00260872) / Mol. Gen. Genet. (1989)
Karginov, F. V., and G. J. Hannon. 2010. The CRISPR system: small RNA-guided defense in bacteria and archaea. Mol. Cell 37 : 7-19. (10.1016/j.molcel.2009.12.033) / Mol. Cell (2010)
Lillestol, R. K., P. Redder, R. A. Garrett, and K. Brugger. 2006. A putative viral defence mechanism in archaeal cells. Archaea 2 : 59-72. (10.1155/2006/542818) / Archaea (2006)
10.1128/JB.183.19.5788-5792.2001
Marraffini, L. A., and E. J. Sontheimer. 2010. Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature 463 : 568-571. (10.1038/nature08703) / Nature (2010)
Meile, L., U. Jenal, D. Studer, M. Jordan, and T. Leisinger. 1989. Characterization of psiM1, a virulent phage of Methanobacterium thermoautotrophicum Marburg. Arch. Microbiol. 152 : 105-110. (10.1007/BF00456085) / Arch. Microbiol. (1989)
Mojica, F. J., C. Diez-Villasenor, J. Garcia-Martinez, and E. Soria. 2005. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J. Mol. Evol. 60 : 174-182. (10.1007/s00239-004-0046-3) / J. Mol. Evol. (2005)
Needleman, S. B., and C. D. Wunsch. 1970. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol. 48 : 443-453. (10.1016/0022-2836(70)90057-4) / J. Mol. Biol. (1970)
Nölling, J., A. Groffen, and W. M. Devos. 1993. phiF1 and phiF3, 2 novel virulent, archaeal phages infecting different thermophilic strains of the genus Methanobacterium. J. Gen. Microbiol. 139 : 2511-2516. (10.1099/00221287-139-10-2511) / J. Gen. Microbiol. (1993)
10.1128/JB.179.22.7135-7155.1997
10.1099/00221287-144-9-2377
Thauer, R. K., A. K. Kaster, M. Goenrich, M. Schick, T. Hiromoto, and S. Shima. 2010. Hydrogenases from methanogenic archaea, nickel, a novel cofactor, and H2 storage. Annu. Rev. Biochem. 79 : 507-536. (10.1146/annurev.biochem.030508.152103) / Annu. Rev. Biochem. (2010)
Thauer, R. K., A. K. Kaster, H. Seedorf, W. Buckel, and R. Hedderich. 2008. Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6 : 579-591. (10.1038/nrmicro1931) / Nat. Rev. Microbiol. (2008)
Wasserfallen, A., J. Nölling, P. Pfister, J. Reeve, and E. Conway de Macario. 2000. Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. Int. J. Syst. Evol. Microbiol. 1 : 43-53. / Int. J. Syst. Evol. Microbiol. (2000)
10.1128/jb.109.2.707-713.1972

Dates

Type	When
Created	15 years ago (Aug. 27, 2010, 10:14 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:14 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 6, 2025, 8:06 a.m.)
Issued	14 years, 10 months ago (Nov. 1, 2010)
Published	14 years, 10 months ago (Nov. 1, 2010)
Published Print	14 years, 10 months ago (Nov. 1, 2010)

Funders 0

None

BibTeX

@article{Liesegang_2010, title={Complete Genome Sequence of Methanothermobacter marburgensis , a Methanoarchaeon Model Organism}, volume={192}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00844-10}, DOI={10.1128/jb.00844-10}, number={21}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Liesegang, Heiko and Kaster, Anne-Kristin and Wiezer, Arnim and Goenrich, Meike and Wollherr, Antje and Seedorf, Henning and Gottschalk, Gerhard and Thauer, Rudolf K.}, year={2010}, month=nov, pages={5850–5851} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T12:06:53Z",
    "timestamp": 1754482013227
  },
  "reference-count": 20,
  "publisher": "American Society for Microbiology",
  "issue": "21",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2010,
            11,
            1
          ]
        ],
        "date-time": "2010-11-01T00:00:00Z",
        "timestamp": 1288569600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2010,
        11
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The circular genome sequence of the chemolithoautotrophic euryarchaeon\n            <jats:italic>Methanothermobacter marburgensis</jats:italic>\n            , with 1,639,135 bp, was determined and compared with that of\n            <jats:italic>Methanothermobacter thermautotrophicus</jats:italic>\n            . The genomes of the two model methanogens differ substantially in protein coding sequences, in insertion sequence (IS)-like elements, and in clustered regularly interspaced short palindromic repeats (CRISPR) loci.\n          </jats:p>",
  "DOI": "10.1128/jb.00844-10",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2010,
        8,
        28
      ]
    ],
    "date-time": "2010-08-28T02:14:50Z",
    "timestamp": 1282961690000
  },
  "page": "5850-5851",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 31,
  "title": "Complete Genome Sequence of\n            <i>Methanothermobacter marburgensis</i>\n            , a Methanoarchaeon Model Organism",
  "prefix": "10.1128",
  "volume": "192",
  "author": [
    {
      "given": "Heiko",
      "family": "Liesegang",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Go\u0308ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Go\u0308ttingen, Germany"
        }
      ]
    },
    {
      "given": "Anne-Kristin",
      "family": "Kaster",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany"
        }
      ]
    },
    {
      "given": "Arnim",
      "family": "Wiezer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Go\u0308ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Go\u0308ttingen, Germany"
        }
      ]
    },
    {
      "given": "Meike",
      "family": "Goenrich",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany"
        }
      ]
    },
    {
      "given": "Antje",
      "family": "Wollherr",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Go\u0308ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Go\u0308ttingen, Germany"
        }
      ]
    },
    {
      "given": "Henning",
      "family": "Seedorf",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany"
        }
      ]
    },
    {
      "given": "Gerhard",
      "family": "Gottschalk",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Go\u0308ttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Go\u0308ttingen, Germany"
        }
      ]
    },
    {
      "given": "Rudolf K.",
      "family": "Thauer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "363",
      "DOI": "10.1093/nar/18.2.363",
      "volume": "18",
      "year": "1990",
      "unstructured": "Bokranz, M., A. Klein, and L. Meile. 1990. Complete nucleotide sequence of plasmid pME2001 of Methanobacterium thermoautotrophicum (Marburg). Nucleic Acids Res. 18 : 363.",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "e_1_3_2_3_2",
      "first-page": "35",
      "year": "1993",
      "unstructured": "Boone, D. R., W. B. Whitman, and P. Rouvi\u00e8re. 1993. Diversity and taxonomy of methanogens, p. 35-80. In J. G. Ferry (ed.), Methanogenesis. Chapman & Hall, New York, NY.",
      "journal-title": "Methanogenesis."
    },
    {
      "key": "e_1_3_2_4_2",
      "first-page": "311",
      "volume": "2",
      "year": "1981",
      "unstructured": "Brandis, A., R. K. Thauer, and K. O. Stetter. 1981. Relatedness of strains DH and Marburg of Methanobacterium thermoautotrophicum. Zentralbl. Bakt. Hyg. I Abt. Orig. C 2 : 311-317.",
      "journal-title": "Zentralbl. Bakt. Hyg. I Abt. Orig. C"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "crossref",
      "first-page": "355",
      "DOI": "10.1146/annurev.bi.59.070190.002035",
      "volume": "59",
      "year": "1990",
      "unstructured": "Dimarco, A. A., T. A. Bobik, and R. S. Wolfe. 1990. Unusual coenzymes of methanogenesis. Annu. Rev. Biochem. 59 : 355-394.",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1007/BF00689352",
      "volume": "117",
      "year": "1978",
      "unstructured": "Fuchs, G., E. Stupperich, and R. K. Thauer. 1978. Acetate assimilation and the synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum. Arch. Microbiol. 117 : 61-66.",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "crossref",
      "first-page": "161",
      "DOI": "10.1007/BF00260872",
      "volume": "220",
      "year": "1989",
      "unstructured": "Jordan, M., L. Meile, and T. Leisinger. 1989. Organization of Methanobacterium thermoautotrophicum bacteriophage psi M1 DNA. Mol. Gen. Genet. 220 : 161-164.",
      "journal-title": "Mol. Gen. Genet."
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "crossref",
      "first-page": "7",
      "DOI": "10.1016/j.molcel.2009.12.033",
      "volume": "37",
      "year": "2010",
      "unstructured": "Karginov, F. V., and G. J. Hannon. 2010. The CRISPR system: small RNA-guided defense in bacteria and archaea. Mol. Cell 37 : 7-19.",
      "journal-title": "Mol. Cell"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "crossref",
      "first-page": "59",
      "DOI": "10.1155/2006/542818",
      "volume": "2",
      "year": "2006",
      "unstructured": "Lillestol, R. K., P. Redder, R. A. Garrett, and K. Brugger. 2006. A putative viral defence mechanism in archaeal cells. Archaea 2 : 59-72.",
      "journal-title": "Archaea"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.183.19.5788-5792.2001"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "568",
      "DOI": "10.1038/nature08703",
      "volume": "463",
      "year": "2010",
      "unstructured": "Marraffini, L. A., and E. J. Sontheimer. 2010. Self versus non-self discrimination during CRISPR RNA-directed immunity. Nature 463 : 568-571.",
      "journal-title": "Nature"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "crossref",
      "first-page": "105",
      "DOI": "10.1007/BF00456085",
      "volume": "152",
      "year": "1989",
      "unstructured": "Meile, L., U. Jenal, D. Studer, M. Jordan, and T. Leisinger. 1989. Characterization of psiM1, a virulent phage of Methanobacterium thermoautotrophicum Marburg. Arch. Microbiol. 152 : 105-110.",
      "journal-title": "Arch. Microbiol."
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "crossref",
      "first-page": "174",
      "DOI": "10.1007/s00239-004-0046-3",
      "volume": "60",
      "year": "2005",
      "unstructured": "Mojica, F. J., C. Diez-Villasenor, J. Garcia-Martinez, and E. Soria. 2005. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J. Mol. Evol. 60 : 174-182.",
      "journal-title": "J. Mol. Evol."
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "crossref",
      "first-page": "443",
      "DOI": "10.1016/0022-2836(70)90057-4",
      "volume": "48",
      "year": "1970",
      "unstructured": "Needleman, S. B., and C. D. Wunsch. 1970. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol. 48 : 443-453.",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "crossref",
      "first-page": "2511",
      "DOI": "10.1099/00221287-139-10-2511",
      "volume": "139",
      "year": "1993",
      "unstructured": "N\u00f6lling, J., A. Groffen, and W. M. Devos. 1993. phiF1 and phiF3, 2 novel virulent, archaeal phages infecting different thermophilic strains of the genus Methanobacterium. J. Gen. Microbiol. 139 : 2511-2516.",
      "journal-title": "J. Gen. Microbiol."
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.179.22.7135-7155.1997"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/00221287-144-9-2377"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "507",
      "DOI": "10.1146/annurev.biochem.030508.152103",
      "volume": "79",
      "year": "2010",
      "unstructured": "Thauer, R. K., A. K. Kaster, M. Goenrich, M. Schick, T. Hiromoto, and S. Shima. 2010. Hydrogenases from methanogenic archaea, nickel, a novel cofactor, and H2 storage. Annu. Rev. Biochem. 79 : 507-536.",
      "journal-title": "Annu. Rev. Biochem."
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "crossref",
      "first-page": "579",
      "DOI": "10.1038/nrmicro1931",
      "volume": "6",
      "year": "2008",
      "unstructured": "Thauer, R. K., A. K. Kaster, H. Seedorf, W. Buckel, and R. Hedderich. 2008. Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6 : 579-591.",
      "journal-title": "Nat. Rev. Microbiol."
    },
    {
      "key": "e_1_3_2_20_2",
      "first-page": "43",
      "volume": "1",
      "year": "2000",
      "unstructured": "Wasserfallen, A., J. N\u00f6lling, P. Pfister, J. Reeve, and E. Conway de Macario. 2000. Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov. Int. J. Syst. Evol. Microbiol. 1 : 43-53.",
      "journal-title": "Int. J. Syst. Evol. Microbiol."
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.109.2.707-713.1972"
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JB.00844-10",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/JB.00844-10",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:14:22Z",
    "timestamp": 1627578862000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/JB.00844-10"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2010,
        11
      ]
    ]
  },
  "references-count": 20,
  "journal-issue": {
    "issue": "21",
    "published-print": {
      "date-parts": [
        [
          2010,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/JB.00844-10"
  ],
  "URL": "http://dx.doi.org/10.1128/JB.00844-10",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        2010,
        11
      ]
    ]
  },
  "assertion": [
    {
      "value": "2010-07-19",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2010-08-13",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2020-12-20",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}