DOI: 10.1128/jb.00701-08. The <i>Anabaena</i> sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions

The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions

10.1128/jb.00701-08

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT The genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 harbors 14 genes containing a GGDEF diguanylate cyclase domain. We found that inactivation of one of these genes, all2874, caused abnormal heterocyst development. The all2874 mutant showed a pronounced reduction in heterocyst frequency during diazotrophic growth and reduced vegetative cell size compared to the wild type. The severity of the mutant phenotype varied with light intensity; at high light intensity, the mutant phenotype was accentuated, whereas at low light intensity the phenotype was similar to wild type. Under high-light growth conditions, the initial heterocyst frequency and pattern for the all2874 mutant were normal, but within 4 days following nitrogen step-down, many intervals between heterocysts increased to as many as 200 vegetative cells, whereas in the wild type the intervals were less than 25 vegetative cells. Filaments containing these unusually long vegetative cell intervals between heterocysts also contained intervals of normal length. An all2874 mutant strain carrying a P patS - gfp transcriptional reporter fusion failed to show normal upregulation of the reporter, which indicates that the decrease in heterocyst frequency is due to an early block in differentiation before induction of the patS gene, which in the wild type takes place 8 h after nitrogen step-down. Genetic epistasis experiments suggest that All2874 acts upstream of the master regulator HetR in differentiating cells. We also showed that purified All2874 functions as a diguanylate cyclase in vitro. We hypothesize that All2874 is required for the normal regulation of heterocyst frequency under high-light growth conditions.

Bibliography

Neunuebel, M. R., & Golden, J. W. (2008). The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions. Journal of Bacteriology, 190(20), 6829â6836.

Authors 2

M. Ramona Neunuebel (first)
James W. Golden (additional)

References 32 Referenced 35

Dates

Type	When
Created	17 years ago (Aug. 22, 2008, 8:45 p.m.)
Deposited	4 years ago (July 29, 2021, 1:42 p.m.)
Indexed	1 year, 1 month ago (July 22, 2024, 5:13 a.m.)
Issued	16 years, 10 months ago (Oct. 15, 2008)
Published	16 years, 10 months ago (Oct. 15, 2008)
Published Print	16 years, 10 months ago (Oct. 15, 2008)

Funders 0

None

BibTeX

@article{Neunuebel_2008, title={The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions}, volume={190}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00701-08}, DOI={10.1128/jb.00701-08}, number={20}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Neunuebel, M. Ramona and Golden, James W.}, year={2008}, month=oct, pages={6829–6836} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            The genome of the heterocyst-forming cyanobacterium\n            <jats:italic>Anabaena</jats:italic>\n            sp. strain PCC 7120 harbors 14 genes containing a GGDEF diguanylate cyclase domain. We found that inactivation of one of these genes, all2874, caused abnormal heterocyst development. The all2874 mutant showed a pronounced reduction in heterocyst frequency during diazotrophic growth and reduced vegetative cell size compared to the wild type. The severity of the mutant phenotype varied with light intensity; at high light intensity, the mutant phenotype was accentuated, whereas at low light intensity the phenotype was similar to wild type. Under high-light growth conditions, the initial heterocyst frequency and pattern for the all2874 mutant were normal, but within 4 days following nitrogen step-down, many intervals between heterocysts increased to as many as 200 vegetative cells, whereas in the wild type the intervals were less than 25 vegetative cells. Filaments containing these unusually long vegetative cell intervals between heterocysts also contained intervals of normal length. An all2874 mutant strain carrying a P\n            <jats:sub>\n              <jats:italic>patS</jats:italic>\n            </jats:sub>\n            -\n            <jats:italic>gfp</jats:italic>\n            transcriptional reporter fusion failed to show normal upregulation of the reporter, which indicates that the decrease in heterocyst frequency is due to an early block in differentiation before induction of the\n            <jats:italic>patS</jats:italic>\n            gene, which in the wild type takes place 8 h after nitrogen step-down. Genetic epistasis experiments suggest that All2874 acts upstream of the master regulator HetR in differentiating cells. We also showed that purified All2874 functions as a diguanylate cyclase in vitro. We hypothesize that All2874 is required for the normal regulation of heterocyst frequency under high-light growth conditions.\n          </jats:p>",
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