DOI: 10.1128/jb.00294-09. The CheZ Binding Interface of CheA <sub>S</sub> Is Located in α-Helix E

The CheZ Binding Interface of CheA S Is Located in α-Helix E

10.1128/jb.00294-09

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

ABSTRACT Specific CheA-short (CheA S ) residues, L123 and L126, were identified as critical for CheZ binding. In the CheA S ′P1-CheZ nuclear magnetic resonance structure, these residues form an interaction surface on α-helix E in the ′P1 domain. Both L123 and L126 are buried in CheA-long (CheA L ), providing an explanation for why CheA L fails to bind CheZ.

Bibliography

OâConnor, C., Matsumura, P., & Campos, A. (2009). The CheZ Binding Interface of CheA S Is Located in Î±-Helix E. Journal of Bacteriology, 191(18), 5845â5848.

Authors 3

Christopher O'Connor (first)
Philip Matsumura (additional)
Andres Campos (additional)

References 20 Referenced 7

Abramoff, M. D., P. J. Magelhaes, and S. J. Ram. 2004. Image processing with ImageJ. Biophotonics Int. 11 : 36-42. / Biophotonics Int. (2004)
10.1128/JB.185.7.2354-2361.2003
Dowd, J. P., and P. Matsumura. 1997. The use of flash photolysis for a high-resolution temporal and spatial analysis of bacterial chemotactic behaviour: CheZ is not always necessary for chemotaxis. Mol. Microbiol. 25 : 295-302. (10.1046/j.1365-2958.1997.4631830.x) / Mol. Microbiol. (1997)
Guex, N., and M. C. Peitsch. 1997. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18 : 2714-2723. (10.1002/elps.1150181505) / Electrophoresis (1997)
10.1128/JB.00323-09
Kentner, D., and V. Sourjik. 2009. Dynamic map of protein interactions in the Escherichia coli chemotaxis pathway. Mol. Syst. Biol. 5 : 238. (10.1038/msb.2008.77) / Mol. Syst. Biol. (2009)
Khan, S., F. Castellano, J. L. Spudich, J. A. McCray, R. S. Goody, and G. P. Reid. 1993. Excitatory signaling in bacteria probed by caged chemoeffectors. Biophys. J. 65 : 2368-2382. (10.1016/S0006-3495(93)81317-1) / Biophys. J. (1993)
10.1128/jb.173.6.2116-2119.1991
10.1128/jb.132.3.996-1002.1977
O'Connor, C., and P. Matsumura. 2004. The accessibility of Cys-120 in CheAS is important for the binding of CheZ and enhancement of CheZ phosphatase activity. Biochemistry 43 : 6909-6916. (10.1021/bi035592n) / Biochemistry (2004)
10.1128/jb.151.1.106-113.1982
Rasband W. S. 2008. ImageJ. National Institutes of Health Bethesda MD. http://rsb.info.nih.gov/ij/ .
Sager, B. M., J. J. Sekelsky, P. Matsumura, and J. Adler. 1988. Use of a computer to assay motility in bacteria. Anal. Biochem. 173 : 271-277. (10.1016/0003-2697(88)90189-3) / Anal. Biochem. (1988)
10.1128/jb.177.10.2713-2720.1995
10.1128/jb.163.2.586-594.1985
Smith, R. A., and J. S. Parkinson. 1980. Overlapping genes at the cheA locus of Escherichia coli. Proc. Natl. Acad. Sci. USA 77 : 5370-5374. (10.1073/pnas.77.9.5370) / Proc. Natl. Acad. Sci. USA (1980)
Sourjik, V., and H. C. Berg. 2000. Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions. Mol. Microbiol. 37 : 740-751. (10.1046/j.1365-2958.2000.02044.x) / Mol. Microbiol. (2000)
10.1128/jb.176.14.4210-4218.1994
Vaknin, A., and H. C. Berg. 2004. Single-cell FRET imaging of phosphatase activity in the Escherichia coli chemotaxis system. Proc. Natl. Acad. Sci. USA 101 : 17072-17077. (10.1073/pnas.0407812101) / Proc. Natl. Acad. Sci. USA (2004)
Wang, H., and P. Matsumura. 1996. Characterization of the CheAS/CheZ complex: a specific interaction resulting in enhanced dephosphorylating activity on CheY-phosphate. Mol. Microbiol. 19 : 695-703. (10.1046/j.1365-2958.1996.393934.x) / Mol. Microbiol. (1996)

Dates

Type	When
Created	16 years, 1 month ago (July 6, 2009, 9:06 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:09 p.m.)
Indexed	2 years, 8 months ago (Dec. 23, 2022, 7:21 p.m.)
Issued	15 years, 11 months ago (Sept. 15, 2009)
Published	15 years, 11 months ago (Sept. 15, 2009)
Published Print	15 years, 11 months ago (Sept. 15, 2009)

Funders 0

None

BibTeX

@article{O_Connor_2009, title={The CheZ Binding Interface of CheA S Is Located in α-Helix E}, volume={191}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00294-09}, DOI={10.1128/jb.00294-09}, number={18}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={O’Connor, Christopher and Matsumura, Philip and Campos, Andres}, year={2009}, month=sep, pages={5845–5848} }

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