Genome Sequence of the Streptomycin-Producing MicroorganismStreptomyces griseusIFO 13350
10.1128/jb.00204-08
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACTWe determined the complete genome sequence ofStreptomyces griseusIFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5′-GGA-3′, are different from those of typical telomeres conserved among otherStreptomycesspecies. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species,Streptomyces coelicolorA3(2) andStreptomyces avermitilis, clarified not only the characteristics of theS. griseusgenome but also the existence of 24Streptomyces-specific proteins. TheS. griseusgenome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a γ-butyrolactone signaling molecule) regulatory cascade inS. griseus.

Bibliography

Ohnishi, Y., Ishikawa, J., Hara, H., Suzuki, H., Ikenoya, M., Ikeda, H., Yamashita, A., Hattori, M., & Horinouchi, S. (2008). Genome Sequence of the Streptomycin-Producing MicroorganismStreptomyces griseusIFO 13350. Journal of Bacteriology, 190(11), 4050–4060.

Authors 9
  1. Yasuo Ohnishi (first)
  2. Jun Ishikawa (additional)
  3. Hirofumi Hara (additional)
  4. Hirokazu Suzuki (additional)
  5. Miwa Ikenoya (additional)
  6. Haruo Ikeda (additional)
  7. Atsushi Yamashita (additional)
  8. Masahira Hattori (additional)
  9. Sueharu Horinouchi (additional)
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Dates
Type When
Created 17 years, 5 months ago (March 29, 2008, 7:14 a.m.)
Deposited 2 years, 3 months ago (May 17, 2023, 5:50 a.m.)
Indexed 14 hours, 15 minutes ago (Sept. 4, 2025, 9:48 a.m.)
Issued 17 years, 3 months ago (June 1, 2008)
Published 17 years, 3 months ago (June 1, 2008)
Published Print 17 years, 3 months ago (June 1, 2008)
Funders 0

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@article{Ohnishi_2008, title={Genome Sequence of the Streptomycin-Producing MicroorganismStreptomyces griseusIFO 13350}, volume={190}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00204-08}, DOI={10.1128/jb.00204-08}, number={11}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ohnishi, Yasuo and Ishikawa, Jun and Hara, Hirofumi and Suzuki, Hirokazu and Ikenoya, Miwa and Ikeda, Haruo and Yamashita, Atsushi and Hattori, Masahira and Horinouchi, Sueharu}, year={2008}, month=jun, pages={4050–4060} }