PilMNOPQ from the Pseudomonas aeruginosa Type IV Pilus System Form a Transenvelope Protein Interaction Network That Interacts with PilA
10.1128/jb.00032-13
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

ABSTRACT Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly and a form of bacterial movement, called twitching motility, that is required for pathogenicity. The type II membrane proteins, PilN and PilO, dimerize via their periplasmic domains and anchor this complex in the inner membrane. Our earlier work showed that PilNO binds PilP, a periplasmic lipoprotein (S. Tammam, L. M. Sampaleanu, J. Koo, P. Sundaram, M. Ayers, P. A. Chong, J. D. Forman-Kay, L. L. Burrows, and P. L. Howell, Mol. Microbiol. 82: 1496–1514, 2011). Here, we show that PilP interacts with the N0 segment of the outer membrane secretin PilQ via its C-terminal domain, and that the N-terminal cytoplasmic tail of PilN binds to the actin-like protein PilM, thereby connecting all cellular compartments via the PilMNOPQ protein interaction network. We show that PilA, the major pilin subunit, interacts with PilNOPQ. The results allow us to propose a model whereby PilA makes extensive contacts with the transenvelope complex, possibly to increase local concentrations of PilA monomers for polymerization. The PilNOP complex could provide a stable anchor in the inner membrane, while the PilMNOPQ transenvelope complex facilitates transit of the pilus through the periplasm and clamps the pilus in the cell envelope. The PilMN interaction is proposed to be responsible for communicating signals from the cytoplasmic to periplasmic components of this complex macromolecular machine.

Bibliography

Tammam, S., Sampaleanu, L. M., Koo, J., Manoharan, K., Daubaras, M., Burrows, L. L., & Howell, P. L. (2013). PilMNOPQ from the Pseudomonas aeruginosa Type IV Pilus System Form a Transenvelope Protein Interaction Network That Interacts with PilA. Journal of Bacteriology, 195(10), 2126–2135.

Authors 7
  1. Stephanie Tammam (first)
  2. Liliana M. Sampaleanu (additional)
  3. Jason Koo (additional)
  4. Kumararaaj Manoharan (additional)
  5. Mark Daubaras (additional)
  6. Lori L. Burrows (additional)
  7. P. Lynne Howell (additional)
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Dates
Type When
Created 12 years, 5 months ago (March 2, 2013, 7:16 a.m.)
Deposited 4 years ago (July 29, 2021, 1:31 p.m.)
Indexed 11 months, 4 weeks ago (Aug. 26, 2024, 7:43 p.m.)
Issued 12 years, 3 months ago (May 15, 2013)
Published 12 years, 3 months ago (May 15, 2013)
Published Print 12 years, 3 months ago (May 15, 2013)
Funders 0

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@article{Tammam_2013, title={PilMNOPQ from the Pseudomonas aeruginosa Type IV Pilus System Form a Transenvelope Protein Interaction Network That Interacts with PilA}, volume={195}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.00032-13}, DOI={10.1128/jb.00032-13}, number={10}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Tammam, Stephanie and Sampaleanu, Liliana M. and Koo, Jason and Manoharan, Kumararaaj and Daubaras, Mark and Burrows, Lori L. and Howell, P. Lynne}, year={2013}, month=may, pages={2126–2135} }