DOI: 10.1128/iai.74.1.488-496.2006. Role of the<i>luxS</i>Quorum-Sensing System in Biofilm Formation and Virulence of<i>Staphylococcus epidermidis</i>

Role of theluxSQuorum-Sensing System in Biofilm Formation and Virulence ofStaphylococcus epidermidis

10.1128/iai.74.1.488-496.2006

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTNosocomial infections caused byStaphylococcus epidermidisare characterized by biofilm formation on implanted medical devices. Quorum-sensing regulation plays a major role in the biofilm development of many bacterial pathogens. Here, we describeluxS, a quorum-sensing system in staphylococci that has a significant impact on biofilm development and virulence. We constructed an isogenicΔluxSmutant strain of a biofilm-forming clinical isolate ofS. epidermidisand demonstrated thatluxSsignaling is functional inS. epidermidis. The mutant strain showed increased biofilm formation in vitro and enhanced virulence in a rat model of biofilm-associated infection. Genetic complementation and addition of autoinducer 2-containing culture filtrate restored the wild-type phenotype, demonstrating thatluxSrepressed biofilm formation through a cell-cell signaling mechanism based on autoinducer 2 secretion. Enhanced production of the biofilm exopolysaccharide polysaccharide intercellular adhesin in the mutant strain is presumably the major cause of the observed phenotype. Theagrquorum-sensing system has previously been shown to impact biofilm development and biofilm-associated infection in a way similar to that ofluxS, although by regulation of different factors. Our study indicates a general scheme of quorum-sensing regulation of biofilm development in staphylococci, which contrasts with that observed in many other bacterial pathogens.

Bibliography

Xu, L., Li, H., Vuong, C., Vadyvaloo, V., Wang, J., Yao, Y., Otto, M., & Gao, Q. (2006). Role of theluxSQuorum-Sensing System in Biofilm Formation and Virulence ofStaphylococcus epidermidis. Infection and Immunity, 74(1), 488â496.

Authors 8

Lin Xu (first)
Hualin Li (additional)
Cuong Vuong (additional)
Viveka Vadyvaloo (additional)
Jianping Wang (additional)
Yufeng Yao (additional)
Michael Otto (additional)
Qian Gao (additional)

References 58 Referenced 189

Dates

Type	When
Created	19 years, 8 months ago (Dec. 20, 2005, 5:02 p.m.)
Deposited	1 year, 7 months ago (Feb. 1, 2024, 4:15 p.m.)
Indexed	4 days, 1 hour ago (Sept. 3, 2025, 6:51 a.m.)
Issued	19 years, 8 months ago (Jan. 1, 2006)
Published	19 years, 8 months ago (Jan. 1, 2006)
Published Print	19 years, 8 months ago (Jan. 1, 2006)

Funders 0

None

BibTeX

@article{Xu_2006, title={Role of theluxSQuorum-Sensing System in Biofilm Formation and Virulence ofStaphylococcus epidermidis}, volume={74}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.74.1.488-496.2006}, DOI={10.1128/iai.74.1.488-496.2006}, number={1}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Xu, Lin and Li, Hualin and Vuong, Cuong and Vadyvaloo, Viveka and Wang, Jianping and Yao, Yufeng and Otto, Michael and Gao, Qian}, year={2006}, month=jan, pages={488–496} }

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