DOI: 10.1128/iai.73.4.2147-2156.2005. Surfactant Protein D Is Present in Human Tear Fluid and the Cornea and Inhibits Epithelial Cell Invasion by<i>Pseudomonas aeruginosa</i>

Surfactant Protein D Is Present in Human Tear Fluid and the Cornea and Inhibits Epithelial Cell Invasion byPseudomonas aeruginosa

10.1128/iai.73.4.2147-2156.2005

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTWe have previously shown that human tear fluid protects corneal epithelial cells againstPseudomonas aeruginosain vitro and in vivo and that protection does not depend upon tear bacteriostatic activity. We sought to identify the responsible tear component(s). The hypothesis tested was that collectins (collagenous calcium-dependent lectins) were involved. Reflex tear fluid was collected from healthy human subjects and examined for collectin content by enzyme-linked immunosorbent assay (ELISA) and Western blot with antibody against surfactant protein D (SP-D), SP-A, or mannose-binding lectin (MBL). SP-D, but not SP-A or MBL, was detected by ELISA of human reflex tear fluid. Western blot analysis of whole tears and of high-performance liquid chromatography tear fractions confirmed the presence of SP-D, most of which eluted in the same fraction as immunoglobulin A. SP-D tear concentrations were calculated at ∼2 to 5 μg/ml. Depletion of SP-D with mannan-conjugated Sepharose or anti-SP-D antibody reduced the protective effect of tears againstP. aeruginosainvasion. Recombinant human or mouse SP-D used alone reducedP. aeruginosainvasion of epithelial cells without detectable bacteriostatic activity or bacterial aggregation. Immunofluorescence microscopy revealed SP-D antibody labeling throughout the corneal epithelium of normal, but not gene-targeted SP-D knockout mice. SP-D was also detected in vitro in cultured human and mouse corneal epithelial cells. In conclusion, SP-D is present in human tear fluid and in human and mouse corneal epithelia. SP-D is involved in human tear fluid protection againstP. aeruginosainvasion. Whether SP-D plays other roles in the regulation of other innate or adaptive immune responses at the ocular surface, as it does in the airways, remains to be explored.

Bibliography

Ni, M., Evans, D. J., Hawgood, S., Anders, E. M., Sack, R. A., & Fleiszig, S. M. J. (2005). Surfactant Protein D Is Present in Human Tear Fluid and the Cornea and Inhibits Epithelial Cell Invasion byPseudomonas aeruginosa. Infection and Immunity, 73(4), 2147â2156.

Authors 6

Minjian Ni (first)
David J. Evans (additional)
Samuel Hawgood (additional)
E. Margot Anders (additional)
Robert A. Sack (additional)
Suzanne M. J. Fleiszig (additional)

References 41 Referenced 75

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Dates

Type	When
Created	20 years, 5 months ago (March 21, 2005, 10:57 p.m.)
Deposited	2 years, 3 months ago (May 2, 2023, 1:34 p.m.)
Indexed	1 month ago (July 24, 2025, 7:47 a.m.)
Issued	20 years, 4 months ago (April 1, 2005)
Published	20 years, 4 months ago (April 1, 2005)
Published Print	20 years, 4 months ago (April 1, 2005)

Funders 0

None

BibTeX

@article{Ni_2005, title={Surfactant Protein D Is Present in Human Tear Fluid and the Cornea and Inhibits Epithelial Cell Invasion byPseudomonas aeruginosa}, volume={73}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.73.4.2147-2156.2005}, DOI={10.1128/iai.73.4.2147-2156.2005}, number={4}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Ni, Minjian and Evans, David J. and Hawgood, Samuel and Anders, E. Margot and Sack, Robert A. and Fleiszig, Suzanne M. J.}, year={2005}, month=apr, pages={2147–2156} }

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