DOI: 10.1128/iai.72.7.3769-3776.2004. Adaptation of <i>Campylobacter jejuni</i> NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract

10.1128/iai.72.7.3769-3776.2004

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT The genome sequence of the human pathogen Campylobacter jejuni NCTC11168 has been determined recently, but studies on colonization and persistence in chickens have been limited due to reports that this strain is a poor colonizer. Experimental colonization and persistence studies were carried out with C. jejuni NCTC11168 by using 2-week-old Light Sussex chickens possessing an acquired natural gut flora. After inoculation, NCTC11168 initially colonized the intestine poorly. However, after 5 weeks we observed adaptation to high-level colonization, which was maintained after in vitro passage. The adapted strain exhibited greatly increased motility. A second strain, C. jejuni 11168H, which had been selected under in vitro conditions for increased motility (A. V. Karlyshev, D. Linton, N. A. Gregson, and B. W. Wren, Microbiology 148: 473-480, 2002), also showed high-level intestinal colonization. The levels of colonization were equivalent to those of six other strains, assessed under the same conditions. There were four mutations in C. jejuni 11168H that reduced colonization; maf5 , flaA (motility and flagellation), and kpsM (capsule deficiency) eliminated colonization, whereas pglH (general glycosylation system deficient) reduced but did not eliminate colonization. This study showed that there was colonization of the avian intestinal tract by a Campylobacter strain having a known genome sequence, and it provides a model for colonization and persistence studies with specific mutations.

Bibliography

Jones, M. A., Marston, K. L., Woodall, C. A., Maskell, D. J., Linton, D., Karlyshev, A. V., Dorrell, N., Wren, B. W., & Barrow, P. A. (2004). Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract. Infection and Immunity, 72(7), 3769â3776.

Authors 9

Michael A. Jones (first)
Kerrie L. Marston (additional)
Claire A. Woodall (additional)
Duncan J. Maskell (additional)
Dennis Linton (additional)
Andrey V. Karlyshev (additional)
Nick Dorrell (additional)
Brendan W. Wren (additional)
Paul A. Barrow (additional)

References 46 Referenced 157

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Dates

Type	When
Created	21 years, 2 months ago (June 22, 2004, 6:43 p.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 12:12 a.m.)
Indexed	1 week, 6 days ago (Aug. 20, 2025, 8:40 a.m.)
Issued	21 years, 2 months ago (July 1, 2004)
Published	21 years, 2 months ago (July 1, 2004)
Published Print	21 years, 2 months ago (July 1, 2004)

Funders 0

None

BibTeX

@article{Jones_2004, title={Adaptation of Campylobacter jejuni NCTC11168 to High-Level Colonization of the Avian Gastrointestinal Tract}, volume={72}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.72.7.3769-3776.2004}, DOI={10.1128/iai.72.7.3769-3776.2004}, number={7}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Jones, Michael A. and Marston, Kerrie L. and Woodall, Claire A. and Maskell, Duncan J. and Linton, Dennis and Karlyshev, Andrey V. and Dorrell, Nick and Wren, Brendan W. and Barrow, Paul A.}, year={2004}, month=jul, pages={3769–3776} }

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