DOI: 10.1128/iai.72.1.154-158.2004. Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells

Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells

10.1128/iai.72.1.154-158.2004

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Apical membrane antigen 1 (AMA-1) of Plasmodium merozoites is established as a candidate molecule for inclusion in a human malaria vaccine and is strongly conserved in the genus. We have investigated its function in merozoite invasion by incubating Plasmodium knowlesi merozoites with red cells in the presence of a previously described rat monoclonal antibody (MAb R31C2) raised against an invasion-inhibitory epitope of P. knowlesi AMA-1 and then fixing the material for ultrastructural analysis. We have found that the random, initial, long-range (12 nm) contact between merozoites and red cells occurs normally in the presence of the antibody, showing that AMA-1 plays no part in this stage of attachment. Instead, inhibited merozoites fail to reorientate, so they do not bring their apices to bear on the red cell surface and do not make close junctional apical contact. We conclude that AMA-1 may be directly responsible for reorientation or that the molecule may initiate the junctional contact, which is then presumably dependent on Duffy binding proteins for its completion.

Bibliography

Mitchell, G. H., Thomas, A. W., Margos, G., Dluzewski, A. R., & Bannister, L. H. (2004). Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells. Infection and Immunity, 72(1), 154â158.

Authors 5

G. H. Mitchell (first)
A. W. Thomas (additional)
G. Margos (additional)
A. R. Dluzewski (additional)
L. H. Bannister (additional)

References 39 Referenced 180

10.1016/0092-8674(90)90295-P
10.1073/pnas.89.15.7085
10.1083/jcb.77.1.72
10.1016/S1471-4922(03)00086-2
10.1017/S0031182000047247
Bannister, L. H., G. A. Butcher, E. D. Dennis, and G. H. Mitchell. 1975. Studies on the structure and invasive behaviour of merozoites of Plasmodium knowlesi. Trans. R. Soc. Trop. Med. Hyg.69:5. / Trans. R. Soc. Trop. Med. Hyg. (1975)
10.1242/jcs.00665
10.1111/j.1550-7408.1989.tb05527.x
Bannister, L. H., G. H. Mitchell, G. A. Butcher, E. D. Dennis, and S. Cohen. 1986. Structure and development of the surface coat of erythrocytic merozoites of Plasmodium knowlesi.Cell Tissue Res.245:281-290. / Cell Tissue Res. (1986)
10.1046/j.1462-5822.1999.00023.x
10.4269/ajtmh.1994.51.711
Deans, J. A., T. Alderson, A. W. Thomas, G. H. Mitchell, E. S. Lennox, and S. Cohen. 1982. Rat monoclonal antibodies which inhibit the in vitro multiplication of Plasmodium knowlesi. Clin. Exp. Immunol.49:297-309. / Clin. Exp. Immunol. (1982)
10.1111/j.1365-3024.1988.tb00241.x
10.1016/0166-6851(84)90065-3
10.1128/IAI.70.6.3101-3110.2002
10.1126/science.803712
10.1016/S0166-6851(01)00326-7
10.1016/S0166-6851(02)00123-8
10.1016/0014-4894(83)90027-9
10.1128/IAI.70.10.5751-5758.2002
10.1074/jbc.M103076200
10.1074/jbc.M207985200
10.1084/jem.149.1.172
10.1038/415673a
Mitchell, G. H., and L. H. Bannister. 1988. Malaria parasite invasion: interactions with the red cell membrane. Crit. Rev. Oncol. Hematol.8:225-310. / Crit. Rev. Oncol. Hematol. (1988)
10.1128/IAI.68.5.2899-2906.2000
10.1016/0166-6851(94)90096-5
10.1128/mcb.9.7.3151-3154.1989
10.1016/S0169-4758(00)01664-1
10.1242/jcs.111.13.1831
10.1073/pnas.96.24.14067
10.1038/415694a
10.1111/j.1365-3024.1990.tb00940.x
10.1016/0166-6851(84)90112-9
10.1590/S0074-02761994000600016
10.1046/j.1365-2958.2000.02175.x
10.4269/ajtmh.2001.65.100
10.1016/S0264-410X(00)00185-7
10.1016/S0021-9258(18)38259-0

Dates

Type	When
Created	21 years, 8 months ago (Dec. 19, 2003, 9:48 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 11:52 p.m.)
Indexed	3 months, 1 week ago (May 26, 2025, 4:05 a.m.)
Issued	21 years, 8 months ago (Jan. 1, 2004)
Published	21 years, 8 months ago (Jan. 1, 2004)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Mitchell_2004, title={Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells}, volume={72}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.72.1.154-158.2004}, DOI={10.1128/iai.72.1.154-158.2004}, number={1}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Mitchell, G. H. and Thomas, A. W. and Margos, G. and Dluzewski, A. R. and Bannister, L. H.}, year={2004}, month=jan, pages={154–158} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        26
      ]
    ],
    "date-time": "2025-05-26T08:05:14Z",
    "timestamp": 1748246714216
  },
  "reference-count": 39,
  "publisher": "American Society for Microbiology",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            1,
            1
          ]
        ],
        "date-time": "2004-01-01T00:00:00Z",
        "timestamp": 1072915200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        1
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Apical membrane antigen 1 (AMA-1) of\n            <jats:italic>Plasmodium</jats:italic>\n            merozoites is established as a candidate molecule for inclusion in a human malaria vaccine and is strongly conserved in the genus. We have investigated its function in merozoite invasion by incubating\n            <jats:italic>Plasmodium knowlesi</jats:italic>\n            merozoites with red cells in the presence of a previously described rat monoclonal antibody (MAb R31C2) raised against an invasion-inhibitory epitope of\n            <jats:italic>P. knowlesi</jats:italic>\n            AMA-1 and then fixing the material for ultrastructural analysis. We have found that the random, initial, long-range (12 nm) contact between merozoites and red cells occurs normally in the presence of the antibody, showing that AMA-1 plays no part in this stage of attachment. Instead, inhibited merozoites fail to reorientate, so they do not bring their apices to bear on the red cell surface and do not make close junctional apical contact. We conclude that AMA-1 may be directly responsible for reorientation or that the molecule may initiate the junctional contact, which is then presumably dependent on Duffy binding proteins for its completion.\n          </jats:p>",
  "DOI": "10.1128/iai.72.1.154-158.2004",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        12,
        20
      ]
    ],
    "date-time": "2003-12-20T02:48:22Z",
    "timestamp": 1071888502000
  },
  "page": "154-158",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 180,
  "title": "Apical Membrane Antigen 1, a Major Malaria Vaccine Candidate, Mediates the Close Attachment of Invasive Merozoites to Host Red Blood Cells",
  "prefix": "10.1128",
  "volume": "72",
  "author": [
    {
      "given": "G. H.",
      "family": "Mitchell",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"
        }
      ]
    },
    {
      "given": "A. W.",
      "family": "Thomas",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Parasitology, Biomedical Primate Research Centre, 2280 GH, Rijswijk, The Netherlands"
        }
      ]
    },
    {
      "given": "G.",
      "family": "Margos",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"
        }
      ]
    },
    {
      "given": "A. R.",
      "family": "Dluzewski",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Immunobiology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London SE1 9RT"
        }
      ]
    },
    {
      "given": "L. H.",
      "family": "Bannister",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Anatomy, Cell and Human Biology, Guy's, King's and St. Thomas' School of Biomedical Science, Guy's Hospital, London SE1 1UL, United Kingdom"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(90)90295-P"
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.15.7085"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.77.1.72"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1471-4922(03)00086-2"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S0031182000047247"
    },
    {
      "key": "e_1_3_2_7_2",
      "first-page": "5",
      "volume": "69",
      "year": "1975",
      "unstructured": "Bannister, L. H., G. A. Butcher, E. D. Dennis, and G. H. Mitchell. 1975. Studies on the structure and invasive behaviour of merozoites of Plasmodium knowlesi. Trans. R. Soc. Trop. Med. Hyg.69:5.",
      "journal-title": "Trans. R. Soc. Trop. Med. Hyg."
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.00665"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1550-7408.1989.tb05527.x"
    },
    {
      "key": "e_1_3_2_10_2",
      "first-page": "281",
      "volume": "245",
      "year": "1986",
      "unstructured": "Bannister, L. H., G. H. Mitchell, G. A. Butcher, E. D. Dennis, and S. Cohen. 1986. Structure and development of the surface coat of erythrocytic merozoites of Plasmodium knowlesi.Cell Tissue Res.245:281-290.",
      "journal-title": "Cell Tissue Res."
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1462-5822.1999.00023.x"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.4269/ajtmh.1994.51.711"
    },
    {
      "key": "e_1_3_2_13_2",
      "first-page": "297",
      "volume": "49",
      "year": "1982",
      "unstructured": "Deans, J. A., T. Alderson, A. W. Thomas, G. H. Mitchell, E. S. Lennox, and S. Cohen. 1982. Rat monoclonal antibodies which inhibit the in vitro multiplication of Plasmodium knowlesi. Clin. Exp. Immunol.49:297-309.",
      "journal-title": "Clin. Exp. Immunol."
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-3024.1988.tb00241.x"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-6851(84)90065-3"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/IAI.70.6.3101-3110.2002"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.803712"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0166-6851(01)00326-7"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0166-6851(02)00123-8"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4894(83)90027-9"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/IAI.70.10.5751-5758.2002"
    },
    {
      "key": "e_1_3_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M103076200"
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.M207985200"
    },
    {
      "key": "e_1_3_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.149.1.172"
    },
    {
      "key": "e_1_3_2_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/415673a"
    },
    {
      "key": "e_1_3_2_26_2",
      "first-page": "225",
      "volume": "8",
      "year": "1988",
      "unstructured": "Mitchell, G. H., and L. H. Bannister. 1988. Malaria parasite invasion: interactions with the red cell membrane. Crit. Rev. Oncol. Hematol.8:225-310.",
      "journal-title": "Crit. Rev. Oncol. Hematol."
    },
    {
      "key": "e_1_3_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/IAI.68.5.2899-2906.2000"
    },
    {
      "key": "e_1_3_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-6851(94)90096-5"
    },
    {
      "key": "e_1_3_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/mcb.9.7.3151-3154.1989"
    },
    {
      "key": "e_1_3_2_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0169-4758(00)01664-1"
    },
    {
      "key": "e_1_3_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.111.13.1831"
    },
    {
      "key": "e_1_3_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.24.14067"
    },
    {
      "key": "e_1_3_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/415694a"
    },
    {
      "key": "e_1_3_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-3024.1990.tb00940.x"
    },
    {
      "key": "e_1_3_2_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-6851(84)90112-9"
    },
    {
      "key": "e_1_3_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1590/S0074-02761994000600016"
    },
    {
      "key": "e_1_3_2_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2000.02175.x"
    },
    {
      "key": "e_1_3_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.4269/ajtmh.2001.65.100"
    },
    {
      "key": "e_1_3_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0264-410X(00)00185-7"
    },
    {
      "key": "e_1_3_2_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)38259-0"
    }
  ],
  "container-title": "Infection and Immunity",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/IAI.72.1.154-158.2004",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/IAI.72.1.154-158.2004",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        5
      ]
    ],
    "date-time": "2022-03-05T04:52:02Z",
    "timestamp": 1646455922000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/IAI.72.1.154-158.2004"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        1
      ]
    ]
  },
  "references-count": 39,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2004,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/IAI.72.1.154-158.2004"
  ],
  "URL": "http://dx.doi.org/10.1128/IAI.72.1.154-158.2004",
  "relation": {},
  "ISSN": [
    "0019-9567",
    "1098-5522"
  ],
  "subject": [],
  "container-title-short": "Infect Immun",
  "published": {
    "date-parts": [
      [
        2004,
        1
      ]
    ]
  },
  "assertion": [
    {
      "value": "2003-07-21",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2003-09-29",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2004-01-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}