DOI: 10.1128/iai.70.9.4826-4832.2002. <i>virB</i>-Mediated Survival of<i>Brucella abortus</i>in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

virB-Mediated Survival ofBrucella abortusin Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

10.1128/iai.70.9.4826-4832.2002

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTTheBrucella abortus virBlocus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whethervirBis involved in evasion of the bactericidal activity of NADPH oxidase and the inducible nitric oxide synthase (iNOS) in macrophages. Elimination of NADPH oxidase or iNOS activity in macrophages in vitro increased recovery of wild-typeB. abortusbut not recovery of avirBmutant. In mice lacking either NADPH oxidase or iNOS, however,B. abortusinfected and persisted to the same extent as it did in congenic C57BL/6 mice up until 60 days postinfection, suggesting that these host defense mechanisms are not critical for limiting bacterial growth in the mouse. AvirBmutant did not exhibit increased survival in either of the knockout mouse strains, indicating that this locus does not contribute to evasion of nitrosative or oxidative killing mechanisms in vivo.

Bibliography

Sun, Y.-H., den Hartigh, A. B., Santos, R. de L., Adams, L. G., & Tsolis, R. M. (2002). virB-Mediated Survival ofBrucella abortusin Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages. Infection and Immunity, 70(9), 4826â4832.

Authors 5

Yao-Hui Sun (first)
Andreas B. den Hartigh (additional)
Renato de Lima Santos (additional)
L. Garry Adams (additional)
Renée M. Tsolis (additional)

References 41 Referenced 0

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Dates

Type	When
Created	22 years, 10 months ago (Oct. 1, 2002, 3:36 p.m.)
Deposited	8 months, 3 weeks ago (Dec. 10, 2024, 12:55 a.m.)
Indexed	8 months, 2 weeks ago (Dec. 11, 2024, 5:10 a.m.)
Issued	22 years, 11 months ago (Sept. 1, 2002)
Published	22 years, 11 months ago (Sept. 1, 2002)
Published Print	22 years, 11 months ago (Sept. 1, 2002)

Funders 0

None

BibTeX

@article{Sun_2002, title={virB-Mediated Survival ofBrucella abortusin Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages}, volume={70}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.70.9.4826-4832.2002}, DOI={10.1128/iai.70.9.4826-4832.2002}, number={9}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Sun, Yao-Hui and den Hartigh, Andreas B. and Santos, Renato de Lima and Adams, L. Garry and Tsolis, Renée M.}, year={2002}, month=sep, pages={4826–4832} }

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