DOI: 10.1128/iai.70.7.3793-3803.2002. <i>Chlamydia trachomatis</i> Induces Remodeling of the Actin Cytoskeleton during Attachment and Entry into HeLa Cells

Chlamydia trachomatis Induces Remodeling of the Actin Cytoskeleton during Attachment and Entry into HeLa Cells

10.1128/iai.70.7.3793-3803.2002

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT To elucidate the host cell machinery utilized by Chlamydia trachomatis to invade epithelial cells, we examined the role of the actin cytoskeleton in the internalization of chlamydial elementary bodies (EBs). Treatment of HeLa cells with cytochalasin D markedly inhibited the internalization of C. trachomatis serovar L2 and D EBs. Association of EBs with HeLa cells induced localized actin polymerization at the site of attachment, as visualized by either phalloidin staining of fixed cells or the active recruitment of GFP-actin in viable infected cells. The recruitment of actin to the specific site of attachment was accompanied by dramatic changes in the morphology of cell surface microvilli. Ultrastructural studies revealed a transient microvillar hypertrophy that was dependent upon C. trachomatis attachment, mediated by structural components on the EBs, and cytochalasin D sensitive. In addition, a mutant CHO cell line that does not support entry of C. trachomatis serovar L2 did not display such microvillar hypertrophy following exposure to L2 EBs, which is in contrast to infection with serovar D, to which it is susceptible. We propose that C. trachomatis entry is facilitated by an active actin remodeling process that is induced by the attachment of this pathogen, resulting in distinct microvillar reorganization throughout the cell surface and the formation of a pedestal-like structure at the immediate site of attachment and entry.

Bibliography

Carabeo, R. A., Grieshaber, S. S., Fischer, E., & Hackstadt, T. (2002). Chlamydia trachomatis Induces Remodeling of the Actin Cytoskeleton during Attachment and Entry into HeLa Cells. Infection and Immunity, 70(7), 3793â3803.

Authors 4

Reynaldo A. Carabeo (first)
Scott S. Grieshaber (additional)
Elizabeth Fischer (additional)
Ted Hackstadt (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:09 a.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 10:02 p.m.)
Indexed	3 months, 1 week ago (May 27, 2025, 10:58 a.m.)
Issued	23 years, 2 months ago (July 1, 2002)
Published	23 years, 2 months ago (July 1, 2002)
Published Print	23 years, 2 months ago (July 1, 2002)

Funders 0

None

BibTeX

@article{Carabeo_2002, title={Chlamydia trachomatis Induces Remodeling of the Actin Cytoskeleton during Attachment and Entry into HeLa Cells}, volume={70}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.70.7.3793-3803.2002}, DOI={10.1128/iai.70.7.3793-3803.2002}, number={7}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Carabeo, Reynaldo A. and Grieshaber, Scott S. and Fischer, Elizabeth and Hackstadt, Ted}, year={2002}, month=jul, pages={3793–3803} }

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