DOI: 10.1128/iai.70.6.3199-3207.2002. Apoptosis in Acute Shigellosis Is Associated with Increased Production of Fas/Fas Ligand, Perforin, Caspase-1, and Caspase-3 but Reduced Production of Bcl-2 and Interleukin-2

Apoptosis in Acute Shigellosis Is Associated with Increased Production of Fas/Fas Ligand, Perforin, Caspase-1, and Caspase-3 but Reduced Production of Bcl-2 and Interleukin-2

10.1128/iai.70.6.3199-3207.2002

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTShigella dysenteriaetype 1-induced apoptotic cell death in rectal tissues from patients infected withShigella dysenteriaetype 1 was studied by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) technique and annexin V staining. Expression of proteins and cytokines participating in the apoptotic process (caspase-1, caspase-3, Fas [CD95], Fas ligand [Fas-L], perforin, granzyme A, Bax, WAF-1, Bcl-2, interleukin-2 [IL-2], IL-18, and granulocyte-macrophage colony-stimulating factor) in tissue in the acute and convalescent stages of dysentery was quantified at the single-cell level by in situ immunostaining. Apoptotic cell death in the lamina propria was markedly up-regulated at the acute stage (P< 0.05), where an increased number of necrotic cells were also seen. Phenotypic analysis of apoptotic cells revealed that 43% of T cells (CD3), 10% of granulocytes (CD15), and 5% of macrophages (CD56) underwent apoptosis. Increased activity of caspase-1 persisted in the rectum up to 1 month after onset. More-extensive expression of Fas, Fas-L, perforin, caspase-3, and IL-18, but not IL-2, at the acute stage than at the convalescent stage was observed. Increased expression of caspase-3 and IL-18 in tissues with severe inflammation compared to expression in those with mild inflammation was evident, implying a possible role in the perpetuation of inflammation. Significantly reduced cell death during convalescence was associated with a significant up-regulation of Bcl-2, Bax, and WAF-1 expression in the rectum compared to that in the acute phase of infection. Thus, induction of apoptosis at the local site in the early phase ofS. dysenteriaetype 1 infection was associated with a significant up-regulation of Fas/Fas-L and perforin and granzyme A expression and a down-regulation of Bcl-2 and IL-2, which promote cell survival.

Bibliography

Raqib, R., Ekberg, C., Sharkar, P., Bardhan, P. K., Zychlinsky, A., Sansonetti, P. J., & Andersson, J. (2002). Apoptosis in Acute Shigellosis Is Associated with Increased Production of Fas/Fas Ligand, Perforin, Caspase-1, and Caspase-3 but Reduced Production of Bcl-2 and Interleukin-2. Infection and Immunity, 70(6), 3199â3207.

Authors 7

Rubhana Raqib (first)
Caroline Ekberg (additional)
Protim Sharkar (additional)
Pradip K. Bardhan (additional)
Arturo Zychlinsky (additional)
Philippe J. Sansonetti (additional)
Jan Andersson (additional)

References 30 Referenced 52

10.1016/S0167-5699(97)01003-7
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Clerc, P., B. Baudry, and P. J. Sansonetti. 1988. Molecular mechanisms of entry, intracellular multiplication and killing of host cells by shigellae. Curr. Top. Microbiol. Immunol.138:3-13. / Curr. Top. Microbiol. Immunol. (1988)
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Mathan, M. M., and V. I. Mathan. 1986. Ultrastructural pathology of the rectal mucosa in Shigella dysentery. Am. J. Pathol.123:25-38. / Am. J. Pathol. (1986)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:09 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 12:04 p.m.)
Indexed	2 months ago (June 30, 2025, 1:05 a.m.)
Issued	23 years, 3 months ago (June 1, 2002)
Published	23 years, 3 months ago (June 1, 2002)
Published Print	23 years, 3 months ago (June 1, 2002)

Funders 0

None

BibTeX

@article{Raqib_2002, title={Apoptosis in Acute Shigellosis Is Associated with Increased Production of Fas/Fas Ligand, Perforin, Caspase-1, and Caspase-3 but Reduced Production of Bcl-2 and Interleukin-2}, volume={70}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.70.6.3199-3207.2002}, DOI={10.1128/iai.70.6.3199-3207.2002}, number={6}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Raqib, Rubhana and Ekberg, Caroline and Sharkar, Protim and Bardhan, Pradip K. and Zychlinsky, Arturo and Sansonetti, Philippe J. and Andersson, Jan}, year={2002}, month=jun, pages={3199–3207} }

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