DOI: 10.1128/iai.70.11.5990-5996.2002. Role of <i>Vibrio cholerae</i> O139 Surface Polysaccharides in Intestinal Colonization

Role of Vibrio cholerae O139 Surface Polysaccharides in Intestinal Colonization

10.1128/iai.70.11.5990-5996.2002

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Since the first occurrence of O139 Vibrio cholerae as a cause of cholera epidemics, this serogroup has been investigated intensively, and it has been found that its pathogenicity is comparable to that of O1 El Tor strains. O139 isolates express a thin capsule, composed of a polymer of repeating units structurally identical to the lipopolysaccharide (LPS) O side chain. In this study, we investigated the role of LPS O side chain and capsular polysaccharide (CPS) in intestinal colonization by with genetically engineered mutants. We constructed CPS-negative, CPS/LPS O side chain-negative, and CPS-positive/LPS O side chain-negative mutants. Furthermore, we constructed two mutants with defects in LPS core oligosaccharide (OS) assembly. Loss of LPS O side chain or CPS resulted in a ≈30-fold reduction in colonization of the infant mouse small intestine, indicating that the presence of both LPS O side chain and CPS is important during the colonization process. The strain lacking both CPS and LPS O side chain and a CPS-positive, LPS O side chain-negative core OS mutant were both essentially unable to colonize. To characterize the role of surface polysaccharides in survival in the host intestine, resistance to several antimicrobial substances was investigated in vitro. These investigations revealed that the presence of CPS protects the cell against attack of the complement system and that an intact core OS is necessary for survival in the presence of bile.

Bibliography

Nesper, J., Schild, S., Lauriano, C. M., Kraiss, A., Klose, K. E., & Reidl, J. (2002). Role of Vibrio cholerae O139 Surface Polysaccharides in Intestinal Colonization. Infection and Immunity, 70(11), 5990â5996.

Authors 6

Jutta Nesper (first)
Stefan Schild (additional)
Crystal M. Lauriano (additional)
Anita Kraiss (additional)
Karl E. Klose (additional)
Joachim Reidl (additional)

References 45 Referenced 48

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Hall R. H. F. M. Khambaty M. Kothary and S. P. Keasler. 1993. Non-O1 Vibrio cholerae . Lancet i: 342-430. (10.1016/0140-6736(93)92839-L)
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Dates

Type	When
Created	22 years, 10 months ago (Oct. 14, 2002, 10:36 p.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 9:28 p.m.)
Indexed	4 months, 3 weeks ago (April 11, 2025, 4:10 a.m.)
Issued	22 years, 10 months ago (Nov. 1, 2002)
Published	22 years, 10 months ago (Nov. 1, 2002)
Published Print	22 years, 10 months ago (Nov. 1, 2002)

Funders 0

None

BibTeX

@article{Nesper_2002, title={Role of Vibrio cholerae O139 Surface Polysaccharides in Intestinal Colonization}, volume={70}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.70.11.5990-5996.2002}, DOI={10.1128/iai.70.11.5990-5996.2002}, number={11}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Nesper, Jutta and Schild, Stefan and Lauriano, Crystal M. and Kraiss, Anita and Klose, Karl E. and Reidl, Joachim}, year={2002}, month=nov, pages={5990–5996} }

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