DOI: 10.1128/iai.7.3.487-492.1973. Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls

Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls

10.1128/iai.7.3.487-492.1973

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

A procedure is given for the insolubilization of yeast mannan by entrapment in a polyacrylamide gel. Specific anti-mannan antibodies were separated by immunosorption from all other antibodies of a rabbit antiserum against whole, Formalininactivated Candida utilis cells. Colloidal gold coated with the anti-mannan and anti-non-mannan antibody fractions was used for the ultrastructural visualization of cell wall antigens. A control was performed using normal rabbit serum. Inhibition of anti-mannan antibodies was obtained with mannan oligosaccharides, and none of the antibody fractions showed marking of Saccharomyces cerevisiae cell walls.

Bibliography

Gerber, H., Horisberger, M., & Bauer, H. (1973). Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls. Infection and Immunity, 7(3), 487â492.

Authors 3

H. Gerber (first)
M. Horisberger (additional)
H. Bauer (additional)

References 0 Referenced 18

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 3, 2020, 9:17 a.m.)
Deposited	3 years, 5 months ago (March 5, 2022, 8:16 a.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 4:37 a.m.)
Issued	52 years, 5 months ago (March 1, 1973)
Published	52 years, 5 months ago (March 1, 1973)
Published Print	52 years, 5 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Gerber_1973, title={Immunosorbent for the Isolation of Specific Antibodies Against Mannan: Localization of Antigens in Yeast Cell Walls}, volume={7}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.7.3.487-492.1973}, DOI={10.1128/iai.7.3.487-492.1973}, number={3}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Gerber, H. and Horisberger, M. and Bauer, H.}, year={1973}, month=mar, pages={487–492} }

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