DOI: 10.1128/iai.69.4.2180-2189.2001. Spa33, a Cell Surface-Associated Subunit of the Mxi-Spa Type III Secretory Pathway of <i>Shigella flexneri</i> , Regulates Ipa Protein Traffic

Spa33, a Cell Surface-Associated Subunit of the Mxi-Spa Type III Secretory Pathway of Shigella flexneri , Regulates Ipa Protein Traffic

10.1128/iai.69.4.2180-2189.2001

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT The Mxi-Spa type III secretion system of Shigella flexneri directs the host cell contact-induced secretion of a set of invasins, referred to as Ipas. In this study, we examined the role of Spa33 in Ipa secretion. A spa33 -null mutant was both noninvasive and unable to translocate the Ipas from inner membrane to outer membrane (OM) positions of the Mxi-Spa transmembrane channel. Spa33 was found to be a Mxi-Spa substrate that is translocated to the bacterial cell surface upon the induction of Ipa secretion. This mobility may serve to drive Ipa translocation within Mxi-Spa toward OM positions. Consistent with a second distinct role in regulating Ipa traffic, the overexpression of Spa33 also blocked Ipa secretion and resulted in Ipa accumulation at the OM. Co-overexpression of Spa33 and another OM-associated element, Spa32, did not disrupt Ipa secretion, suggesting an interaction between the two proteins and an effect on the mechanism which serves to regulate Ipa release from the OM. These findings indicate that Spa33 is a mobile element within Mxi-Spa, which is required to control Ipa translocation into and out of OM positions of the secretory structure.

Bibliography

Schuch, R., & Maurelli, A. T. (2001). Spa33, a Cell Surface-Associated Subunit of the Mxi-Spa Type III Secretory Pathway of Shigella flexneri , Regulates Ipa Protein Traffic. Infection and Immunity, 69(4), 2180â2189.

Authors 2

Raymond Schuch (first)
Anthony T. Maurelli (additional)

References 49 Referenced 29

Dates

Type	When
Created	23 years ago (July 27, 2002, 6:01 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 9:19 p.m.)
Indexed	1 year ago (July 27, 2024, 9:11 a.m.)
Issued	24 years, 4 months ago (April 1, 2001)
Published	24 years, 4 months ago (April 1, 2001)
Published Print	24 years, 4 months ago (April 1, 2001)

Funders 0

None

BibTeX

@article{Schuch_2001, title={Spa33, a Cell Surface-Associated Subunit of the Mxi-Spa Type III Secretory Pathway of Shigella flexneri , Regulates Ipa Protein Traffic}, volume={69}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.69.4.2180-2189.2001}, DOI={10.1128/iai.69.4.2180-2189.2001}, number={4}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Schuch, Raymond and Maurelli, Anthony T.}, editor={DiRita, V. J.}, year={2001}, month=apr, pages={2180–2189} }

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