DOI: 10.1128/iai.69.2.737-743.2001. <i>Salmonella</i> Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System

Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System

10.1128/iai.69.2.737-743.2001

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Survival of Salmonella enterica serovar Typhimurium within host phagocytic cells is a critical step in establishing systemic infection in mice. Genes within Salmonella pathogenicity island 2 (SPI-2) encode a type III secretion system that is required for establishment of systemic infection. Several proteins encoded by SPI-2 have homology to type III secreted proteins from enteropathogenic Escherichia coli and Yersinia and, based on that homology, are predicted to be secreted through the SPI-2 type III secretion system. We have investigated the roles of two of these proteins, SseC and SseD. We demonstrate here that the SseD protein is required for systemic Salmonella infection of the mouse, and we confirmed the virulence requirement for the SseC protein. Experiments were performed, using cellular fractionation and immunoblotting, to identify the subcellular location of the SseC and SseD proteins. Both proteins were found to localize predominantly to the bacterial cell membrane. In addition, our work revealed that SseC and SseD are exposed to the extracellular environment and are loosely associated with the bacterial membrane. Furthermore, localization of SseC and SseD to the bacterial membrane was found to require a functional SPI-2 type III secretion system. Collectively, these results indicate that the SseC and SseD proteins are secreted by the SPI-2 type III secretion system to the bacterial membrane in order to perform their virulence functions.

Bibliography

Klein, J. R., & Jones, B. D. (2001). Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System. Infection and Immunity, 69(2), 737â743.

Authors 2

Joanna R. Klein (first)
Bradley D. Jones (additional)

References 27 Referenced 46

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 9:02 p.m.)
Indexed	2 months, 2 weeks ago (June 16, 2025, 10:08 a.m.)
Issued	24 years, 7 months ago (Feb. 1, 2001)
Published	24 years, 7 months ago (Feb. 1, 2001)
Published Print	24 years, 7 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Klein_2001, title={Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System}, volume={69}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.69.2.737-743.2001}, DOI={10.1128/iai.69.2.737-743.2001}, number={2}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Klein, Joanna R. and Jones, Bradley D.}, editor={Barbieri, J. T.}, year={2001}, month=feb, pages={737–743} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Survival of\n            <jats:italic>Salmonella enterica</jats:italic>\n            serovar Typhimurium within host phagocytic cells is a critical step in establishing systemic infection in mice. Genes within\n            <jats:italic>Salmonella</jats:italic>\n            pathogenicity island 2 (SPI-2) encode a type III secretion system that is required for establishment of systemic infection. Several proteins encoded by SPI-2 have homology to type III secreted proteins from enteropathogenic\n            <jats:italic>Escherichia coli</jats:italic>\n            and\n            <jats:italic>Yersinia</jats:italic>\n            and, based on that homology, are predicted to be secreted through the SPI-2 type III secretion system. We have investigated the roles of two of these proteins, SseC and SseD. We demonstrate here that the SseD protein is required for systemic\n            <jats:italic>Salmonella</jats:italic>\n            infection of the mouse, and we confirmed the virulence requirement for the SseC protein. Experiments were performed, using cellular fractionation and immunoblotting, to identify the subcellular location of the SseC and SseD proteins. Both proteins were found to localize predominantly to the bacterial cell membrane. In addition, our work revealed that SseC and SseD are exposed to the extracellular environment and are loosely associated with the bacterial membrane. Furthermore, localization of SseC and SseD to the bacterial membrane was found to require a functional SPI-2 type III secretion system. Collectively, these results indicate that the SseC and SseD proteins are secreted by the SPI-2 type III secretion system to the bacterial membrane in order to perform their virulence functions.\n          </jats:p>",
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