DOI: 10.1128/iai.69.2.1127-1133.2001. Characterization of Murine Lung Dendritic Cells Infected with<i>Mycobacterium tuberculosis</i>

Characterization of Murine Lung Dendritic Cells Infected withMycobacterium tuberculosis

10.1128/iai.69.2.1127-1133.2001

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTLung dendritic cells were identified by immunohistochemistry in lung tissue sections from C57BL/6 mice. Following isolation from the lungs using CD11c magnetic beads, the flow cytometric analysis of I-Ab+and CD11c+cells indicated a mixed population of dendritic cells at different stages of maturation, with most expressing an immature phenotype. When cultured for 7 days with recombinant murine granulocyte-macrophage colony-stimulating factor, 99% of cells were CD11c+and had a morphology typical of immature dendritic cells. These cells were negative for CD34, CD14, and CD8α antigens but expressed low levels of the myeloid marker F4/80 and moderate levels of MAC3. All expressed high levels of CD11a (LFA-1), CD11b (Mac1), and CD54 antigens, with low levels of class II major histocompatibility complex. Most cells expressed CD80 but only a small percentage of cells were positive for CD40 and CD86. Both overnight and 7-day cultures of lung dendritic cells were able to phagocytoseMycobacterium tuberculosis, and this was associated with the production of interleukin-12 and stimulation of both naı̈ve and immune T cells to produce gamma interferon.

Bibliography

Gonzalez-Juarrero, M., & Orme, I. M. (2001). Characterization of Murine Lung Dendritic Cells Infected withMycobacterium tuberculosis. Infection and Immunity, 69(2), 1127â1133.

Authors 2

Mercedes Gonzalez-Juarrero (first)
Ian M. Orme (additional)

References 28 Referenced 116

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11:45 a.m.)
Indexed	4 months, 3 weeks ago (April 9, 2025, 3:18 a.m.)
Issued	24 years, 7 months ago (Feb. 1, 2001)
Published	24 years, 7 months ago (Feb. 1, 2001)
Published Print	24 years, 7 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Gonzalez_Juarrero_2001, title={Characterization of Murine Lung Dendritic Cells Infected withMycobacterium tuberculosis}, volume={69}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.69.2.1127-1133.2001}, DOI={10.1128/iai.69.2.1127-1133.2001}, number={2}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Gonzalez-Juarrero, Mercedes and Orme, Ian M.}, editor={Barbieri, J. T.}, year={2001}, month=feb, pages={1127–1133} }

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