DOI: 10.1128/iai.69.11.7121-7129.2001. Characterization of Antiapoptotic Activities of<i>Chlamydia pneumoniae</i>in Human Cells

Characterization of Antiapoptotic Activities ofChlamydia pneumoniaein Human Cells

10.1128/iai.69.11.7121-7129.2001

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTChlamydia pneumoniaeis an obligate intracellular bacterium which frequently causes airway infection in humans and has been implicated in atherosclerosis. Here we show that infection withC. pneumoniaeprotects HeLa human epithelioid cells against apoptosis induced by external stimuli. In infected HeLa cells, apoptosis induced by staurosporine and CD95-death-receptor signaling was strongly reduced. Upon treatment with staurosporine, generation of effector caspase activity, processing of caspase-3 and caspase-9 and cytochromecredistribution were all profoundly inhibited in cells infected withC. pneumoniae. Bacterial protein synthesis during early infection was required for this inhibition. Furthermore, cytochromec-induced processing and activation of caspases were inhibited in cytosolic extracts from infected cells, suggesting that aC. pneumoniae-dependent antiapoptotic factor was generated in the cytosol upon infection. Infection withC. pneumoniaefailed to induce significant NF-κB activation in HeLa cells, indicating that no NF-κB-dependent cellular factors were involved in the protection against apoptosis. These results show thatC. pneumoniaeis capable of interfering with the host cell's apoptotic apparatus at probably at least two steps in signal transduction and might explain the propensity of these bacteria to cause chronic infections in humans.

Bibliography

Fischer, S. F., Schwarz, C., Vier, J., & HaÌcker, G. (2001). Characterization of Antiapoptotic Activities ofChlamydia pneumoniaein Human Cells. Infection and Immunity, 69(11), 7121â7129.

Authors 4

Silke F. Fischer (first)
Claudia Schwarz (additional)
Juliane Vier (additional)
Georg Häcker (additional)

References 36 Referenced 97

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11:27 a.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:38 a.m.)
Issued	23 years, 10 months ago (Nov. 1, 2001)
Published	23 years, 10 months ago (Nov. 1, 2001)
Published Print	23 years, 10 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Fischer_2001, title={Characterization of Antiapoptotic Activities ofChlamydia pneumoniaein Human Cells}, volume={69}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.69.11.7121-7129.2001}, DOI={10.1128/iai.69.11.7121-7129.2001}, number={11}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Fischer, Silke F. and Schwarz, Claudia and Vier, Juliane and Häcker, Georg}, editor={Burns, D. L.}, year={2001}, month=nov, pages={7121–7129} }

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