DOI: 10.1128/iai.69.1.405-412.2001. Secreted Aspartic Proteinase Family of <i>Candida tropicalis</i>

Secreted Aspartic Proteinase Family of Candida tropicalis

10.1128/iai.69.1.405-412.2001

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Medically important yeasts of the genus Candida secrete aspartic proteinases (Saps), which are of particular interest as virulence factors. Like Candida albicans , Candida tropicalis secretes in vitro one dominant Sap (Sapt1p) in a medium containing bovine serum albumin (BSA) as the sole source of nitrogen. Using the gene SAPT1 as a probe and under low-stringency hybridization conditions, three new closely related gene sequences, SAPT2 to SAPT4 , encoding secreted proteinases were cloned from a C. tropicalis λEMBL3 genomic library. All bands identified by Southern blotting of Eco RI-digested C. tropicalis genomic DNA with SAPT1 could be assigned to a specific SAP gene. Therefore, the SAPT gene family of C. tropicalis is likely to contain only four members. Interestingly, the SAPT2 and SAPT3 gene products, Sapt2p and Sapt3p, which have not yet been detected in C. tropicalis cultures in vitro, were produced as active recombinant enzymes with the methylotrophic yeast Pichia pastoris as an expression system. As expected, reverse transcriptase PCR experiments revealed a strong SAPT1 signal with RNA extracted from cells grown in BSA medium. However, a weak signal was obtained with all other SAPT genes under several conditions tested, showing that these SAPT genes could be expressed at a basic level. Together, these experiments suggest that the gene products Sapt2p, Sapt3p, and Sapt4p could be produced under conditions yet to be described in vitro or during infection.

Bibliography

Zaugg, C., Borg-von Zepelin, M., Reichard, U., Sanglard, D., & Monod, M. (2001). Secreted Aspartic Proteinase Family of Candida tropicalis. Infection and Immunity, 69(1), 405â412.

Authors 5

Christophe Zaugg (first)
Margarete Borg-von Zepelin (additional)
Utz Reichard (additional)
Dominique Sanglard (additional)
Michel Monod (additional)

References 34 Referenced 101

10.1080/02681219080000021
10.1128/iai.56.3.626-631.1988
10.1046/j.1365-2958.1998.00815.x
10.1046/j.1523-1747.1999.00747.x
10.1016/0003-2697(76)90527-3
10.1016/0378-1119(88)90606-3
10.1086/314546
10.1099/00221287-139-2-335
10.1128/AEM.64.12.4809-4815.1998
10.1093/nar/15.16.6737
10.1093/clinids/7.5.646
10.1002/1097-0061(20000630)16:9<847::AID-YEA562>3.0.CO;2-9
Hube B. Candida albicans secreted aspartyl proteinases.Curr. Top. Med. Mycol.719965569 / Curr. Top. Med. Mycol. / Candida albicans secreted aspartyl proteinases by Hube B. (1996)
10.1093/clinids/11.3.379
10.1099/00222615-13-3-423
10.1099/00221287-144-10-2731
10.1111/j.1365-2958.1994.tb00429.x
10.1128/mcb.12.7.2997-3005.1992
Odds F. C. Candida and candidosis 2nd ed. 1988 Bailliére Tindall London United Kingdom
10.1086/520194
10.1128/iai.53.2.411-419.1986
Ruchel R. Uhlemann K. Boning B. Secretion of acid proteinases by different species of the genus Candida.Zentbl. Bakteriol. Mikrobiol. Hyg. A2551983537548 / Zentbl. Bakteriol. Mikrobiol. Hyg. A / Secretion of acid proteinases by different species of the genus Candida by Ruchel R. (1983)
10.1007/BF01626348
Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y
10.1046/j.1365-2958.1999.01590.x
10.1046/j.1365-2958.1998.00957.x
10.1021/bi970613x
10.1002/jcb.240330106
10.1016/0014-5793(91)80969-A
10.1080/02681219480000331
10.1099/13500872-142-3-493
10.1007/BF01591361
10.1093/nar/14.11.4683
10.1016/S0195-6701(97)90206-1

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 9:05 p.m.)
Indexed	4 months, 2 weeks ago (April 7, 2025, 5:41 a.m.)
Issued	24 years, 7 months ago (Jan. 1, 2001)
Published	24 years, 7 months ago (Jan. 1, 2001)
Published Print	24 years, 7 months ago (Jan. 1, 2001)

Funders 0

None

BibTeX

@article{Zaugg_2001, title={Secreted Aspartic Proteinase Family of Candida tropicalis}, volume={69}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.69.1.405-412.2001}, DOI={10.1128/iai.69.1.405-412.2001}, number={1}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Zaugg, Christophe and Borg-von Zepelin, Margarete and Reichard, Utz and Sanglard, Dominique and Monod, Michel}, editor={Moore, R. N.}, year={2001}, month=jan, pages={405–412} }

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