DOI: 10.1128/iai.68.7.4102-4107.2000. Identification of Genes Required for Chronic Persistence of<i>Brucella abortus</i>in Mice

Identification of Genes Required for Chronic Persistence ofBrucella abortusin Mice

10.1128/iai.68.7.4102-4107.2000

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTThe genetic basis for chronic persistence ofBrucella abortusin lymphoid organs of mice, cows, and humans is currently unknown. We identifiedB. abortusgenes involved in chronic infection, by assessing the ability of 178 signature-tagged mutants to establish and maintain persistent infection in mice. Each mutant was screened for its ability to colonize the spleens of mice at 2 and 8 weeks after inoculation. Comparison of the results from both time points identified two groups of mutants attenuated for chronic infection in mice. The first group was not recovered at either 2 or 8 weeks postinfection and was therefore defective in establishing infection. Mutants in this group carried transposon insertions in genes involved in lipopolysaccharide biosynthesis (wbkA), in aromatic amino acid biosynthesis, and in type IV secretion (virB1andvirB10). The second group, which was recovered at wild-type levels 2 weeks postinfection but not 8 weeks postinfection was able to establish infection but was unable to maintain chronic infection. One mutant in this group carried a transposon insertion in a gene with homology togcvBofMycobacterium tuberculosis, encoding glycine dehydrogenase, an enzyme whose activity is increased during the state of nonreplicating persistence. These results suggest that some mechanisms for long-term persistence may be shared among chronic intracellular pathogens. Furthermore, identification of two groups of genes, those required for initiating infection and those required only for long-term persistence, suggests thatB. abortususes distinct sets of virulence determinants to establish and maintain chronic infection in mice.

Authors 3

Priscilla C. Hong (first)
Renée M. Tsolis (additional)
Thomas A. Ficht (additional)

References 32 Referenced 200

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Alton G. G. Jones L. M. Pietz D. E. Laboratory techniques in brucellosis 2nd ed. 1975 World Health Organization Geneva Switzerland
10.1093/nar/25.17.3389
Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. J. 1994 Wiley & Sons New York N.Y
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Castillo A. Taboada H. Mendoza A. Valderrama B. Encarnacion S. Mora J. GenBank accession number AF107264 Rhizobium etli glutamate synthase large subunit (gltB) and glutamate synthase small subunit (gltD) genes complete cds. 1998
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Eisenschenk F. C. Houle J. J. Hoffmann E. M. Serum sensitivity of field isolates and laboratory strains of Brucella abortus.Am. J. Vet. Res.56199515921598 (10.2460/ajvr.1995.56.12.1592) / Am. J. Vet. Res. / Serum sensitivity of field isolates and laboratory strains of Brucella abortus by Eisenschenk F. C. (1995)
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Gerhardt P. The nutrition of brucellae.Microbiol. Rev.2219588198 / Microbiol. Rev. / The nutrition of brucellae by Gerhardt P. (1958)
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Kovach M. E. Phillips R. W. Elzer P. H. Roop R. M. Peterson K. M. pBBR1MCS: a broad-host-range cloning vector.BioTechniques161994800801 / BioTechniques / pBBR1MCS: a broad-host-range cloning vector by Kovach M. E. (1994)
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Meador V. P. Tabatabai L. B. Hagemoser W. A. Deyoe B. L. Identification of Brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique.Am. J. Vet. Res.47198621472150 / Am. J. Vet. Res. / Identification of Brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique by Meador V. P. (1986)
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Plommet M. Plommet A. M. Évolution de l'infection splénique de souris de quatre lignées, inoculées par voie veneuse, par trois doses de Brucella abortus.Ann. Rech. Vet.121981345351 / Ann. Rech. Vet. / Évolution de l'infection splénique de souris de quatre lignées, inoculées par voie veneuse, par trois doses de Brucella abortus by Plommet M. (1981)
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10.1128/iai.37.3.1042-1049.1982
10.1093/clinids/21.2.283

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	8 months, 3 weeks ago (Dec. 8, 2024, 3:22 p.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 9:30 a.m.)
Issued	25 years, 1 month ago (July 1, 2000)
Published	25 years, 1 month ago (July 1, 2000)
Published Print	25 years, 1 month ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Hong_2000, title={Identification of Genes Required for Chronic Persistence ofBrucella abortusin Mice}, volume={68}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.68.7.4102-4107.2000}, DOI={10.1128/iai.68.7.4102-4107.2000}, number={7}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Hong, Priscilla C. and Tsolis, Renée M. and Ficht, Thomas A.}, editor={O’Brien, A. D.}, year={2000}, month=jul, pages={4102–4107} }

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