DOI: 10.1128/iai.68.7.4049-4054.2000. Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from <i>Bordetella pertussis</i>

Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from Bordetella pertussis

10.1128/iai.68.7.4049-4054.2000

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT We examined the structural components of pertussis toxin that are required for efficient export from Bordetella pertussis via the Ptl system, a member of the type IV family of macromolecular transporters. First, we constructed a strain of B. pertussis that contains a functional Ptl system but does not produce pertussis toxin. Plasmids which express either the S1 subunit or the B oligomer were then introduced into this strain. We found that the B oligomer of the toxin is not secreted in the absence of the S1 subunit. Conversely, the S1 subunit is also not secreted by a Ptl-mediated mechanism in the absence of the B oligomer. Thus, an assembled holotoxin is required for Ptl-mediated export of pertussis toxin from B. pertussis .

Bibliography

Farizo, K. M., Huang, T., & Burns, D. L. (2000). Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from Bordetella pertussis. Infection and Immunity, 68(7), 4049â4054.

Authors 3

Karen M. Farizo (first)
Theresa Huang (additional)
Drusilla L. Burns (additional)

References 32 Referenced 44

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 8:45 p.m.)
Indexed	1 year, 1 month ago (July 17, 2024, 4:28 a.m.)
Issued	25 years, 1 month ago (July 1, 2000)
Published	25 years, 1 month ago (July 1, 2000)
Published Print	25 years, 1 month ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Farizo_2000, title={Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from Bordetella pertussis}, volume={68}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.68.7.4049-4054.2000}, DOI={10.1128/iai.68.7.4049-4054.2000}, number={7}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Farizo, Karen M. and Huang, Theresa and Burns, Drusilla L.}, editor={Barbieri, J. T.}, year={2000}, month=jul, pages={4049–4054} }

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