DOI: 10.1128/iai.68.4.1953-1963.2000. Macrophage Class A Scavenger Receptor-Mediated Phagocytosis of<i>Escherichia coli</i>: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro

Macrophage Class A Scavenger Receptor-Mediated Phagocytosis ofEscherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro

10.1128/iai.68.4.1953-1963.2000

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTMacrophage class A scavenger receptors (SR-AI and SR-AII) contribute to host defense by binding polyanionic ligands such as lipopolysaccharide and lipoteichoic acid. SR-A knockout (SR-A−/−) mice are more susceptible to endotoxic shock andListeria monocytogenesinfection in vivo, possibly due to decreased clearance of lipopolysaccharide and microorganisms, respectively. We have used flow cytometry to analyze the role of SR-A and other scavenger-like receptors in phagocytosis of bacteria in vitro. Chinese hamster ovary cells stably transfected with human SR-A boundEscherichia coliandStaphylococcus aureusbut ingested few organisms. Primary human monocyte-derived macrophages (Mφ) bound and ingestedE. colimore efficiently, and this was partially but selectively blocked by the general SR inhibitor, poly(I). A specific and selective role for SR-A was shown, since bone marrow culture-derived Mφ from SR-A−/−mice ingested fewerE. coliorganisms than did wild-type cells, while uptake of antibody-opsonizedE. coliwas unaffected. SR-A-dependent uptake ofE. colivaried with the bacterial strain; ingestion of DH5α and K1 by SR-A−/−Mφ was reduced by 30 to 60% and 70 to 75%, respectively. Phagocytosis and endocytosis via SR-A were markedly down-modulated when Mφ were plated on serum-coated tissue culture plastic compared to bacteriologic plastic, where cell adhesion is mediated by SR-A and CR3, respectively. This paper demonstrates that SR-A can bind and ingest bacteria directly, consistent with a role in host defense in vivo, and highlights the importance of the source of the Mφ, bacterial strain, and culture conditions on receptor function in vitro.

Bibliography

Peiser, L., Gough, P. J., Kodama, T., & Gordon, S. (2000). Macrophage Class A Scavenger Receptor-Mediated Phagocytosis ofEscherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro. Infection and Immunity, 68(4), 1953â1963.

Authors 4

Leanne Peiser (first)
Peter J. Gough (additional)
Tatsuhiko Kodama (additional)
Siamon Gordon (additional)

References 49 Referenced 185

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:01 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11:16 a.m.)
Indexed	1 month, 1 week ago (July 28, 2025, 5:44 p.m.)
Issued	25 years, 5 months ago (April 1, 2000)
Published	25 years, 5 months ago (April 1, 2000)
Published Print	25 years, 5 months ago (April 1, 2000)

Funders 0

None

BibTeX

@article{Peiser_2000, title={Macrophage Class A Scavenger Receptor-Mediated Phagocytosis ofEscherichia coli: Role of Cell Heterogeneity, Microbial Strain, and Culture Conditions In Vitro}, volume={68}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.68.4.1953-1963.2000}, DOI={10.1128/iai.68.4.1953-1963.2000}, number={4}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Peiser, Leanne and Gough, Peter J. and Kodama, Tatsuhiko and Gordon, Siamon}, editor={Kaufmann, S. H. E.}, year={2000}, month=apr, pages={1953–1963} }

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  ]
}