DOI: 10.1128/iai.68.3.1418-1427.2000. Vaccine Efficacy of Recombinant<i>Plasmodium falciparum</i>Merozoite Surface Protein 1 in Malaria-Naive, -Exposed, and/or -Rechallenged<i>Aotus vociferans</i>Monkeys

Vaccine Efficacy of RecombinantPlasmodium falciparumMerozoite Surface Protein 1 in Malaria-Naive, -Exposed, and/or -RechallengedAotus vociferansMonkeys

10.1128/iai.68.3.1418-1427.2000

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTProtection against a lethal challenge infection ofPlasmodium falciparumwas elicited in malaria-naiveAotus vociferansmonkeys by vaccination with the C terminus 19-kDa protein of the major merozoite surface protein (MSP-119) fused to tetanus toxoid universal T-cell epitopes P30 and P2. Three of four monkeys were protected against a 104-parasite challenge. Four monkeys were challenged with 105parasites; one self-cured the infection, two were protected against high parasitemia (<2%) but were treated for severe anemia (hematocrit of <25%), and the fourth was not protected. In this model system, anemia appears to be a manifestation of incomplete protection (prolonged low-level parasitemia). Enzyme-linked immunosorbent assay (ELISA) antibody titers correlated with protection. Antibodies from some protected monkeys inhibited secondary processing of MSP-142to MSP-133and MSP-119. To mimic the repeated reinfections seen in regions where malaria is endemic, a second malaria parasite challenge was administered 4 months later. All P30P2MSP-119-vaccinated monkeys were protected; thus, a single challenge infection may underestimate vaccine efficacy. ELISA antibody titers correlated with protection against a second infection but had decreased compared to the first challenge. As most target populations for asexual blood-stage malaria vaccines will have been exposed to malaria parasites, a malaria parasite-exposed monkey was vaccinated with P30P2MSP-119. This monkey was completely protected, while a malaria parasite-naive P30P2MSP-119-vaccinated monkey self-cured a low-grade parasitemia. Prior malaria parasite infection primed the production of anti-native MSP-119antibodies, which were boosted by vaccination with recombinant P30P2MSP-119. Preliminary data suggest that immunogenicity studies of vaccines designed for malaria parasite-exposed populations should also be conducted in malaria parasite-exposed subjects.

Bibliography

Egan, A. F., Blackman, M. J., & Kaslow, D. C. (2000). Vaccine Efficacy of RecombinantPlasmodium falciparumMerozoite Surface Protein 1 in Malaria-Naive, -Exposed, and/or -RechallengedAotus vociferansMonkeys. Infection and Immunity, 68(3), 1418â1427.

Authors 3

Andrea F. Egan (first)
Michael J. Blackman (additional)
David C. Kaslow (additional)

References 27 Referenced 58

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Chang S. P. Gibson H. L. Lee-Ng C. T. Barr P. J. Hui G. S. N. A carboxyl-terminal fragment of Plasmodium falciparum gp195 expressed by a recombinant baculovirus induces antibodies that completely inhibit parasite growth.J. Immunol.1491992548555 (10.4049/jimmunol.149.2.548) / J. Immunol. / A carboxyl-terminal fragment of Plasmodium falciparum gp195 expressed by a recombinant baculovirus induces antibodies that completely inhibit parasite growth by Chang S. P. (1992)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:03 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11:44 a.m.)
Indexed	4 months, 4 weeks ago (April 8, 2025, 6:58 p.m.)
Issued	25 years, 6 months ago (March 1, 2000)
Published	25 years, 6 months ago (March 1, 2000)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Egan_2000, title={Vaccine Efficacy of RecombinantPlasmodium falciparumMerozoite Surface Protein 1 in Malaria-Naive, -Exposed, and/or -RechallengedAotus vociferansMonkeys}, volume={68}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.68.3.1418-1427.2000}, DOI={10.1128/iai.68.3.1418-1427.2000}, number={3}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Egan, Andrea F. and Blackman, Michael J. and Kaslow, David C.}, editor={Mansfield, J. M.}, year={2000}, month=mar, pages={1418–1427} }

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