DOI: 10.1128/iai.68.12.6879-6882.2000. Expression of the Nitric Oxide Synthase 2 Gene Is Not Essential for Early Control of<i>Mycobacterium tuberculosis</i>in the Murine Lung

Expression of the Nitric Oxide Synthase 2 Gene Is Not Essential for Early Control ofMycobacterium tuberculosisin the Murine Lung

10.1128/iai.68.12.6879-6882.2000

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTThe interleukin-12 and gamma interferon (IFN-γ) pathway of macrophage activation plays a pivotal role in controlling tuberculosis. In the murine model, the generation of supplementary nitric oxide by the induction of the nitric oxide synthase 2 (NOS2) gene product is considered the principal antimicrobial mechanism of IFN-γ-activated macrophages. Using a low-dose aerosol-mediated infection model in the mouse, we have investigated the role of nitric oxide in controllingMycobacterium tuberculosisin the lung. In contrast to the consequences of a systemic infection, a low dose of bacteria introduced directly into the lungs of mice lacking the NOS2 gene is controlled almost as well as in intact animals. This is in contrast to the rapid progression of disease in mice lacking IFN-γ or a key member of the IFN signaling pathway, interferon regulatory factor 1. Thus while IFN-γ is pivotal in early control of bacterial growth in the lung, this control does not completely depend upon the expression of the NOS2 gene. The absence of inducible nitric oxide in the lung does, however, result in increased polymorphonuclear cell involvement and eventual necrosis in the pulmonary granulomas of the infected mice lacking the NOS2 gene.

Bibliography

Cooper, A. M., Pearl, J. E., Brooks, J. V., Ehlers, S., & Orme, I. M. (2000). Expression of the Nitric Oxide Synthase 2 Gene Is Not Essential for Early Control ofMycobacterium tuberculosisin the Murine Lung. Infection and Immunity, 68(12), 6879â6882.

Authors 5

Andrea M. Cooper (first)
John E. Pearl (additional)
Jason V. Brooks (additional)
Stefan Ehlers (additional)
Ian M. Orme (additional)

References 30 Referenced 112

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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:58 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 11 a.m.)
Indexed	3 months, 2 weeks ago (May 14, 2025, 12:47 a.m.)
Issued	24 years, 9 months ago (Dec. 1, 2000)
Published	24 years, 9 months ago (Dec. 1, 2000)
Published Print	24 years, 9 months ago (Dec. 1, 2000)

Funders 0

None

BibTeX

@article{Cooper_2000, title={Expression of the Nitric Oxide Synthase 2 Gene Is Not Essential for Early Control ofMycobacterium tuberculosisin the Murine Lung}, volume={68}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.68.12.6879-6882.2000}, DOI={10.1128/iai.68.12.6879-6882.2000}, number={12}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Cooper, Andrea M. and Pearl, John E. and Brooks, Jason V. and Ehlers, Stefan and Orme, Ian M.}, editor={Clements, J. D.}, year={2000}, month=dec, pages={6879–6882} }

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