DOI: 10.1128/iai.67.4.1770-1778.1999. <i>Listeria monocytogenes</i>Phospholipase C-Dependent Calcium Signaling Modulates Bacterial Entry into J774 Macrophage-Like Cells [Penn]

Listeria monocytogenesPhospholipase C-Dependent Calcium Signaling Modulates Bacterial Entry into J774 Macrophage-Like Cells

10.1128/iai.67.4.1770-1778.1999

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTListeria monocytogenessecretes several proteins that have been shown to contribute to virulence. Among these is listeriolysin O (LLO), a pore-forming hemolysin that is absolutely required for virulence. Two other virulence factors are phospholipases: a phosphatidylinositol-specific phospholipase C (PI-PLC [plcA]) and a broad-range PLC (plcB). Although mutations inplcAorplcBresulted in small increases in mouse 50% lethal dose (LD50), deletions in both genes resulted in a 500-fold increase in LD50. We have examined the role of these secreted proteins in host intracellular signaling in the J774 macrophage-like cell line. Measurements of cytosolic free calcium ([Ca2+]i) have revealed a rapid spike upon exposure of these cells to wild-typeL. monocytogenes. This is followed by a second peak at 5 min and a third prolonged peak with a maximal [Ca2+]iof 800 to 1,000 nM. The pattern of calcium changes was greatly altered by deletion of any of the three virulence factors. An LLO mutant produced none of these elevations in [Ca2+]i; however, a transient elevation was observed whenever these bacteria entered the cell. A PI-PLC mutant produced a diminished single elevation in [Ca2+]iat 15 to 30 min. A broad-range PLC mutant produced only the first calcium spike. Studies with inhibitors suggested that the first elevation arises from influx of calcium from the extracellular medium through plasma membrane channels and that the second and third elevations come from release of Ca2+from intracellular stores. We observed that internalization of wild-type bacteria and the broad-range PLC mutant was delayed for 5 to 10 min, but the LLO and PI-PLC mutants were internalized rapidly upon infection. Inhibitors that affected calcium signaling changed the kinetics of association of wild-type bacteria with J774 cells, the kinetics of entry, and the efficiency of escape from the primary phagosome.

Bibliography

Wadsworth, S. J., & Goldfine, H. (1999). Listeria monocytogenesPhospholipase C-Dependent Calcium Signaling Modulates Bacterial Entry into J774 Macrophage-Like Cells. Infection and Immunity, 67(4), 1770â1778.

Authors 2

Sandra J. Wadsworth (first)
Howard Goldfine (additional)

References 60 Referenced 91

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Dates

Type	When
Created	5 years, 5 months ago (March 9, 2020, 3:45 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 6:54 a.m.)
Indexed	1 year ago (Aug. 10, 2024, 1:56 a.m.)
Issued	26 years, 4 months ago (April 1, 1999)
Published	26 years, 4 months ago (April 1, 1999)
Published Print	26 years, 4 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Wadsworth_1999, title={Listeria monocytogenesPhospholipase C-Dependent Calcium Signaling Modulates Bacterial Entry into J774 Macrophage-Like Cells}, volume={67}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.67.4.1770-1778.1999}, DOI={10.1128/iai.67.4.1770-1778.1999}, number={4}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Wadsworth, Sandra J. and Goldfine, Howard}, editor={Orndorff, P. E.}, year={1999}, month=apr, pages={1770–1778} }

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