DOI: 10.1128/iai.66.10.4932-4941.1998. Characterization of Group B Streptococcal Invasion of Human Chorion and Amnion Epithelial Cells In Vitro

Characterization of Group B Streptococcal Invasion of Human Chorion and Amnion Epithelial Cells In Vitro

10.1128/iai.66.10.4932-4941.1998

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Group B streptococci (GBS) have been cultured from the chorioamnionic membrane of pregnant women, usually in association with chorioamnionitis and premature labor (K. A. Boggess, D. H. Watts, S. L. Hillier, M. A. Krohn, T. J. Benedetti, and D. A. Eschenbach, Obstet. Gynecol. 87: 779–784, 1996). Colonization and infection of placental membranes can be a prelude to neonatal GBS infections even in the presence of intact membranes (R. L. Naeye and E. C. Peters, Pediatrics 61: 171–177, 1978), suggesting that GBS cause chorioamnionitis or establish amniotic fluid infections by partial or complete penetration of the placental membranes. We have isolated and grown cultures of primary chorion and amnion cells from human cesarean-section placentas. This has provided a biologically relevant model for investigating GBS adherence to and invasion of the two epithelial barriers of the placental membrane. GBS adhered to chorion cell monolayers to a high degree. Pretreatment of GBS with trypsin reduced adherence up to 10-fold, which suggested that the bacterial ligand(s) was a protein. GBS invaded chorion cells at a high rate in vitro, and invasion was dependent on cellular actin polymerization. GBS could be seen within intracellular vacuoles of chorion cells by transmission electron microscopy. We also demonstrated that GBS were capable of transcytosing through intact chorion cell monolayers without disruption of intracellular junctions. GBS also adhered to amnion cells; in contrast, however, these bacteria failed to invade amnion cells under a variety of assay conditions. GBS interactions with the chorion epithelial cell layer shown here correlate well with epidemiological and pathological studies of GBS chorioamnionitis. Our data also suggest that the amnion cell layer may provide an effective barrier against infection of the amniotic fluid.

Bibliography

Winram, S. B., Jonas, M., Chi, E., & Rubens, C. E. (1998). Characterization of Group B Streptococcal Invasion of Human Chorion and Amnion Epithelial Cells In Vitro. Infection and Immunity, 66(10), 4932â4941.

Authors 4

Scott B. Winram (first)
Mechthild Jonas (additional)
Emil Chi (additional)
Craig E. Rubens (additional)

References 48 Referenced 62

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10.1016/0002-9378(87)90070-6
10.1016/0029-7844(96)00037-3
Centers for Disease Control and Prevention Prevention of perinatal group B streptococcal disease: a public health perspective.Morbid. Mortal. Weekly Rep.RR-71996124 / Morbid. Mortal. Weekly Rep. / Prevention of perinatal group B streptococcal disease: a public health perspective by Centers for Disease Control and Prevention (1996)
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10.1016/0029-7844(95)00402-5

Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 10:20 a.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 7:05 p.m.)
Indexed	4 months, 3 weeks ago (April 11, 2025, 8:29 a.m.)
Issued	26 years, 11 months ago (Oct. 1, 1998)
Published	26 years, 11 months ago (Oct. 1, 1998)
Published Print	26 years, 11 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{Winram_1998, title={Characterization of Group B Streptococcal Invasion of Human Chorion and Amnion Epithelial Cells In Vitro}, volume={66}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.66.10.4932-4941.1998}, DOI={10.1128/iai.66.10.4932-4941.1998}, number={10}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Winram, Scott B. and Jonas, Mechthild and Chi, Emil and Rubens, Craig E.}, editor={Fischetti, V. A.}, year={1998}, month=oct, pages={4932–4941} }

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  "relation": {},
  "ISSN": [
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    "1098-5522"
  ],
  "subject": [],
  "container-title-short": "Infect Immun",
  "published": {
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        10
      ]
    ]
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