DOI: 10.1128/iai.26.2.698-704.1979. Serospecificity and opsonic activity of antisera to Legionella pneumophila

Serospecificity and opsonic activity of antisera to Legionella pneumophila

10.1128/iai.26.2.698-704.1979

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

A high-molecular-weight surface component (F-1 fraction) has been isolated from the four serogroups of Legionella pneumophila. Antibody raised against live organisms was found by microagglutination assay to be specific for the homologous serogroup. Agglutinating activity of antiserum was markedly diminished after absorption with the homologous, but not heterologous, F-1 fraction. In addition, it was found that L. pneumophila organisms were not interiorized by rat alveolar macrophages or mouse peritoneal macrophages in the absence of antiserum, whereas homologous antiserum effectively opsonized the organisms. The opsonizing activity of serogroup-specific antisera was eliminated by absorption of the antisera with the homologous, but not heterologous, F-1 fraction. These data indicate that the serogroup-specific antigen of L. pneumophila resides in the F-1 fraction and that antibody to the F-1 fraction is required for phagocytosis of L. pneumophila by mammalian phagocytes.

Bibliography

Johnson, W., Pesanti, E., & Elliott, J. (1979). Serospecificity and opsonic activity of antisera to Legionella pneumophila. Infection and Immunity, 26(2), 698â704.

Authors 3

W Johnson (first)
E Pesanti (additional)
J Elliott (additional)

References 0 Referenced 28

None

Dates

Type	When
Created	5 years, 8 months ago (Jan. 1, 2020, 12:19 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 4:38 p.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 12:53 a.m.)
Issued	45 years, 10 months ago (Nov. 1, 1979)
Published	45 years, 10 months ago (Nov. 1, 1979)
Published Print	45 years, 10 months ago (Nov. 1, 1979)

Funders 0

None

BibTeX

@article{Johnson_1979, title={Serospecificity and opsonic activity of antisera to Legionella pneumophila}, volume={26}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.26.2.698-704.1979}, DOI={10.1128/iai.26.2.698-704.1979}, number={2}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Johnson, W and Pesanti, E and Elliott, J}, year={1979}, month=nov, pages={698–704} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        23
      ]
    ],
    "date-time": "2023-10-23T04:53:25Z",
    "timestamp": 1698036805330
  },
  "reference-count": 0,
  "publisher": "American Society for Microbiology",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1979,
            11,
            1
          ]
        ],
        "date-time": "1979-11-01T00:00:00Z",
        "timestamp": 310262400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        11
      ]
    ]
  },
  "abstract": "<jats:p>A high-molecular-weight surface component (F-1 fraction) has been isolated from the four serogroups of Legionella pneumophila. Antibody raised against live organisms was found by microagglutination assay to be specific for the homologous serogroup. Agglutinating activity of antiserum was markedly diminished after absorption with the homologous, but not heterologous, F-1 fraction. In addition, it was found that L. pneumophila organisms were not interiorized by rat alveolar macrophages or mouse peritoneal macrophages in the absence of antiserum, whereas homologous antiserum effectively opsonized the organisms. The opsonizing activity of serogroup-specific antisera was eliminated by absorption of the antisera with the homologous, but not heterologous, F-1 fraction. These data indicate that the serogroup-specific antigen of L. pneumophila resides in the F-1 fraction and that antibody to the F-1 fraction is required for phagocytosis of L. pneumophila by mammalian phagocytes.</jats:p>",
  "DOI": "10.1128/iai.26.2.698-704.1979",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        1
      ]
    ],
    "date-time": "2020-01-01T05:19:06Z",
    "timestamp": 1577855946000
  },
  "page": "698-704",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 28,
  "title": "Serospecificity and opsonic activity of antisera to Legionella pneumophila",
  "prefix": "10.1128",
  "volume": "26",
  "author": [
    {
      "given": "W",
      "family": "Johnson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "E",
      "family": "Pesanti",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J",
      "family": "Elliott",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "235",
  "container-title": "Infection and Immunity",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/iai.26.2.698-704.1979",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/iai.26.2.698-704.1979",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        3,
        4
      ]
    ],
    "date-time": "2022-03-04T21:38:54Z",
    "timestamp": 1646429934000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/iai.26.2.698-704.1979"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        11
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1979,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/iai.26.2.698-704.1979"
  ],
  "URL": "http://dx.doi.org/10.1128/IAI.26.2.698-704.1979",
  "relation": {},
  "ISSN": [
    "0019-9567",
    "1098-5522"
  ],
  "subject": [],
  "container-title-short": "Infect Immun",
  "published": {
    "date-parts": [
      [
        1979,
        11
      ]
    ]
  },
  "assertion": [
    {
      "value": "1979-11-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}