DOI: 10.1128/iai.00940-09. <i>Klebsiella pneumoniae</i> Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells

10.1128/iai.00940-09

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACT Human β-defensins (hBDs) contribute to the protection of the respiratory tract against pathogens. It is reasonable to postulate that pathogens have developed countermeasures to resist them. Klebsiella pneumoniae capsule polysaccharide (CPS), but not the lipopolysaccharide O antigen, mediated resistance against hBD1 and hBD2. hBD3 was the most potent hBD against Klebsiella . We investigated the possibility that as a strategy for survival in the lung, K. pneumoniae may not activate the expression of hBDs. Infection of A549 and normal human bronchial cells with 52145-Δ wca K2 , a CPS mutant, increased the expression of hBD2 and hBD3. Neither the wild type nor the lipopolysaccharide O antigen mutant increased the expression of hBDs. In vivo , 52145-Δ wca K2 induced higher levels of mBD4 and mBD14, possible mouse orthologues of hBD2 and hBD3, respectively, than the wild type. 52145-Δ wca K2 -dependent upregulation of hBD2 occurred via NF-κB and mitogen-activated protein kinases (MAPKs) p44/42, Jun N-terminal protein kinase (JNK)-dependent pathways. The increase in hBD3 expression was dependent on the MAPK JNK. 52145-Δ wca K2 engaged Toll-like receptors 2 and 4 (TLR2 and TLR4) to activate hBD2, whereas hBD3 expression was dependent on NOD1. K. pneumoniae induced the expression of CYLD and MKP-1, which act as negative regulators for 52145-Δ wca K2 -induced expression of hBDs. Bacterial engagement of pattern recognition receptors induced CYLD and MKP-1, which may initiate the attenuation of proinflammatory pathways. The results of this study indicate that K. pneumoniae CPS not only protects the pathogen from the bactericidal action of defensins but also impedes their expression. These features of K. pneumoniae CPS may facilitate pathogen survival in the hostile environment of the lung.

Bibliography

Moranta, D., Regueiro, V., March, C., Llobet, E., Margareto, J., Larrate, E., Garmendia, J., & Bengoechea, J. A. (2010). Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of Î²-Defensins by Airway Epithelial Cells. Infection and Immunity, 78(3), 1135â1146.

Authors 8

David Moranta (first)
Verónica Regueiro (additional)
Catalina March (additional)
Enrique Llobet (additional)
Javier Margareto (additional)
Eider Larrate (additional)
Junkal Garmendia (additional)
José A. Bengoechea (additional)

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Dates

Type	When
Created	15 years, 8 months ago (Dec. 14, 2009, 9:47 p.m.)
Deposited	3 years, 6 months ago (March 5, 2022, 4:46 a.m.)
Indexed	2 days, 3 hours ago (Sept. 4, 2025, 10:07 a.m.)
Issued	15 years, 6 months ago (March 1, 2010)
Published	15 years, 6 months ago (March 1, 2010)
Published Print	15 years, 6 months ago (March 1, 2010)

Funders 0

None

BibTeX

@article{Moranta_2010, title={Klebsiella pneumoniae Capsule Polysaccharide Impedes the Expression of β-Defensins by Airway Epithelial Cells}, volume={78}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.00940-09}, DOI={10.1128/iai.00940-09}, number={3}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Moranta, David and Regueiro, Verónica and March, Catalina and Llobet, Enrique and Margareto, Javier and Larrate, Eider and Garmendia, Junkal and Bengoechea, José A.}, year={2010}, month=mar, pages={1135–1146} }

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