DOI: 10.1128/iai.00889-09. Inactivation of Phospholipase D Diminishes<i>Acinetobacter baumannii</i>Pathogenesis

Inactivation of Phospholipase D DiminishesAcinetobacter baumanniiPathogenesis

10.1128/iai.00889-09

Crossref journal-article

American Society for Microbiology

Infection and Immunity (235)

Abstract

ABSTRACTAcinetobacter baumanniiis an emerging bacterial pathogen of considerable health care concern. Nonetheless, relatively little is known about the organism's virulence factors or their regulatory networks. Septicemia and ventilator-associated pneumonia are two of the more severe forms ofA. baumanniidisease. To identify virulence factors that may contribute to these disease processes, genetically diverseA. baumanniiclinical isolates were evaluated for the ability to proliferate in human serum. A transposon mutant library was created in a strain background that propagated well in serum and screened for members with decreased serum growth. The results revealed that disruption ofA. baumanniiphospholipase D (PLD) caused a reduction in the organism's ability to thrive in serum, a deficiency in epithelial cell invasion, and diminished pathogenesis in a murine model of pneumonia. Collectively, these results suggest that PLD is anA. baumanniivirulence factor.

Bibliography

Jacobs, A. C., Hood, I., Boyd, K. L., Olson, P. D., Morrison, J. M., Carson, S., Sayood, K., Iwen, P. C., Skaar, E. P., & Dunman, P. M. (2010). Inactivation of Phospholipase D DiminishesAcinetobacter baumanniiPathogenesis. Infection and Immunity, 78(5), 1952â1962.

Authors 10

Anna C. Jacobs (first)
Indriati Hood (additional)
Kelli L. Boyd (additional)
Patrick D. Olson (additional)
John M. Morrison (additional)
Steven Carson (additional)
Khalid Sayood (additional)
Peter C. Iwen (additional)
Eric P. Skaar (additional)
Paul M. Dunman (additional)

References 44 Referenced 181

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Dates

Type	When
Created	15 years, 6 months ago (March 1, 2010, 11:34 p.m.)
Deposited	6 months, 2 weeks ago (Feb. 18, 2025, 5:47 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 20, 2025, 8:57 a.m.)
Issued	15 years, 4 months ago (May 1, 2010)
Published	15 years, 4 months ago (May 1, 2010)
Published Print	15 years, 4 months ago (May 1, 2010)

Funders 0

None

BibTeX

@article{Jacobs_2010, title={Inactivation of Phospholipase D DiminishesAcinetobacter baumanniiPathogenesis}, volume={78}, ISSN={1098-5522}, url={http://dx.doi.org/10.1128/iai.00889-09}, DOI={10.1128/iai.00889-09}, number={5}, journal={Infection and Immunity}, publisher={American Society for Microbiology}, author={Jacobs, Anna C. and Hood, Indriati and Boyd, Kelli L. and Olson, Patrick D. and Morrison, John M. and Carson, Steven and Sayood, Khalid and Iwen, Peter C. and Skaar, Eric P. and Dunman, Paul M.}, year={2010}, month=may, pages={1952–1962} }

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