DOI: 10.1128/ec.3.6.1609-1618.2004. Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the <i>RIM101</i> Pathway in <i>Candida albicans</i>

Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans

10.1128/ec.3.6.1609-1618.2004

Crossref journal-article

American Society for Microbiology

Eukaryotic Cell (235)

Abstract

ABSTRACT The success of Candida albicans as an opportunistic pathogen is based in part on its ability to adapt to diverse environments. The RIM101 pathway governs adaptation to neutral-alkaline environments and is required for virulence. Analysis of a genomic two-hybrid study conducted with Saccharomyces cerevisiae revealed that components involved in multivesicular bodies (MVB) transport may interact with RIM101 pathway members. Thus, we hypothesized that these proteins may function in the RIM101 pathway in C. albicans . We identified C. albicans homologs to S. cerevisiae Snf7p, Vps4p, and Bro1p and generated mutants in the cognate gene. We found that snf7 Δ/Δ mutants, but not vps4 Δ/Δ nor bro1 Δ/Δ mutants, had phenotypes similar to, but more severe than, those of RIM101 pathway mutants. We found that the constitutively active RIM101-405 allele partially rescued snf7 Δ/Δ mutant phenotypes. The vps4 Δ/Δ mutant had subtle phenotypes, but these were not rescued by the RIM101-405 allele. Further, we found that the snf7 Δ/Δ, vps4 Δ/Δ, and bro1 Δ/Δ mutants did not efficiently localize the vital dye FM4-64 to the vacuole and that it was often accumulated in an MVB-like compartment. This phenotype was not rescued by RIM101-405 or observed in RIM101 pathway mutants. These results suggest that Snf7p may serve two functions in the cell: one as a RIM101 pathway member and one for MVB transport to the vacuole.

Bibliography

Kullas, A. L., Li, M., & Davis, D. A. (2004). Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans. Eukaryotic Cell, 3(6), 1609â1618.

Authors 3

Amy L. Kullas (first)
Mingchun Li (additional)
Dana A. Davis (additional)

References 43 Referenced 58

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Mol. Biol. Cell 2004

Dates

Type	When
Created	20 years, 8 months ago (Dec. 8, 2004, 1:39 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 8:31 a.m.)
Indexed	4 months, 3 weeks ago (April 9, 2025, 5:48 a.m.)
Issued	20 years, 8 months ago (Dec. 1, 2004)
Published	20 years, 8 months ago (Dec. 1, 2004)
Published Print	20 years, 8 months ago (Dec. 1, 2004)

Funders 0

None

BibTeX

@article{Kullas_2004, title={Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans}, volume={3}, ISSN={1535-9786}, url={http://dx.doi.org/10.1128/ec.3.6.1609-1618.2004}, DOI={10.1128/ec.3.6.1609-1618.2004}, number={6}, journal={Eukaryotic Cell}, publisher={American Society for Microbiology}, author={Kullas, Amy L. and Li, Mingchun and Davis, Dana A.}, year={2004}, month=dec, pages={1609–1618} }

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