DOI: 10.1128/ec.3.4.984-991.2004. CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in <i>Aspergillus nidulans</i>

CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in Aspergillus nidulans

10.1128/ec.3.4.984-991.2004

Crossref journal-article

American Society for Microbiology

Eukaryotic Cell (235)

Abstract

ABSTRACT The alternation of eukaryotic life cycles between haploid and diploid phases is crucial for maintaining genetic diversity. In some organisms, the growth and development of haploid and diploid phases are nearly identical, and one might suppose that all genes required for one phase are likely to be critical for the other phase. Here, we show that targeted disruption of the chpA (cysteine- and histidine-rich-domain- [CHORD]-containing protein A) gene in haploid Aspergillus nidulans strains gives rise to chpA knockout haploids and heterozygous diploids but no chpA knockout diploids. A. nidulans chpA heterozygous diploids showed impaired conidiophore development and reduced conidiation. Deletion of chpA from diploid A. nidulans resulted in genome instability and reversion to a haploid state. Thus, our data suggest a vital role for chpA in maintenance of the diploid phase in A. nidulans . Furthermore, the human chpA homolog, Chp-1 , was able to complement haploinsufficiency in A. nidulans chpA heterozygotes, suggesting that the function of CHORD-containing proteins is highly conserved in eukaryotes.

Bibliography

Sadanandom, A., Findlay, K., Doonan, J. H., Schulze-Lefert, P., & Shirasu, K. (2004). CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in Aspergillus nidulans. Eukaryotic Cell, 3(4), 984â991.

Authors 5

Ari Sadanandom (first)
Kim Findlay (additional)
John H. Doonan (additional)
Paul Schulze-Lefert (additional)
Ken Shirasu (additional)

References 32 Referenced 7

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Dates

Type	When
Created	21 years ago (Aug. 9, 2004, 4:54 p.m.)
Deposited	3 years, 5 months ago (Feb. 23, 2022, 8:29 a.m.)
Indexed	1 year, 5 months ago (March 2, 2024, 2:31 a.m.)
Issued	21 years ago (Aug. 1, 2004)
Published	21 years ago (Aug. 1, 2004)
Published Print	21 years ago (Aug. 1, 2004)

Funders 0

None

BibTeX

@article{Sadanandom_2004, title={CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in Aspergillus nidulans}, volume={3}, ISSN={1535-9786}, url={http://dx.doi.org/10.1128/ec.3.4.984-991.2004}, DOI={10.1128/ec.3.4.984-991.2004}, number={4}, journal={Eukaryotic Cell}, publisher={American Society for Microbiology}, author={Sadanandom, Ari and Findlay, Kim and Doonan, John H. and Schulze-Lefert, Paul and Shirasu, Ken}, year={2004}, month=aug, pages={984–991} }

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