DOI: 10.1128/ec.3.2.331-338.2004. Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in <i>Saccharomyces cerevisiae</i>

Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae

10.1128/ec.3.2.331-338.2004

Crossref journal-article

American Society for Microbiology

Eukaryotic Cell (235)

Abstract

ABSTRACT When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding.

Bibliography

Stahl, G., Salem, S. N. B., Chen, L., Zhao, B., & Farabaugh, P. J. (2004). Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae. Eukaryotic Cell, 3(2), 331â338.

Authors 5

Guillaume Stahl (first)
Samia N. Ben Salem (additional)
Lifeng Chen (additional)
Bing Zhao (additional)
Philip J. Farabaugh (additional)

References 48 Referenced 42

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Dates

Type	When
Created	21 years, 4 months ago (April 9, 2004, 4:03 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 8:28 a.m.)
Indexed	1 year, 1 month ago (July 18, 2024, 5:45 a.m.)
Issued	21 years, 4 months ago (April 1, 2004)
Published	21 years, 4 months ago (April 1, 2004)
Published Print	21 years, 4 months ago (April 1, 2004)

Funders 0

None

BibTeX

@article{Stahl_2004, title={Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae}, volume={3}, ISSN={1535-9786}, url={http://dx.doi.org/10.1128/ec.3.2.331-338.2004}, DOI={10.1128/ec.3.2.331-338.2004}, number={2}, journal={Eukaryotic Cell}, publisher={American Society for Microbiology}, author={Stahl, Guillaume and Salem, Samia N. Ben and Chen, Lifeng and Zhao, Bing and Farabaugh, Philip J.}, year={2004}, month=apr, pages={331–338} }

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