DOI: 10.1128/ec.1.2.229-240.2002. Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in <i>Saccharomyces cerevisiae</i>

Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae

10.1128/ec.1.2.229-240.2002

Crossref journal-article

American Society for Microbiology

Eukaryotic Cell (235)

Abstract

ABSTRACT Previously, antibodies were raised against a nuclear envelope-enriched fraction of yeast, and the essential gene NNF1 was cloned by reverse genetics. Here it is shown that the conditional nnf1 - 17 mutant has decreased stability of a minichromosome in addition to mitotic spindle defects. I have identified the novel essential genes DSN1, DSN3 , and NSL1 through genetic interactions with nnf1 - 17 . Dsn3p was found to be equivalent to the kinetochore protein Mtw1p. By indirect immunofluorescence, all four proteins, Nnf1p, Mtw1p, Dsn1p, and Nsl1p, colocalize and are found in the region of the spindle poles. Based on the colocalization of these four proteins, the minichromosome instability and the spindle defects seen in nnf1 mutants, I propose that Nnf1p is part of a new group of proteins necessary for chromosome segregation.

Bibliography

Euskirchen, G. M. (2002). Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae. Eukaryotic Cell, 1(2), 229â240.

Authors 1

Ghia M. Euskirchen (first)

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Dates

Type	When
Created	22 years, 8 months ago (Dec. 4, 2002, 8:41 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 7:47 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:07 p.m.)
Issued	23 years, 4 months ago (April 1, 2002)
Published	23 years, 4 months ago (April 1, 2002)
Published Print	23 years, 4 months ago (April 1, 2002)

Funders 0

None

BibTeX

@article{Euskirchen_2002, title={Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae}, volume={1}, ISSN={1535-9786}, url={http://dx.doi.org/10.1128/ec.1.2.229-240.2002}, DOI={10.1128/ec.1.2.229-240.2002}, number={2}, journal={Eukaryotic Cell}, publisher={American Society for Microbiology}, author={Euskirchen, Ghia M.}, year={2002}, month=apr, pages={229–240} }

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