DOI: 10.1128/ec.00004-08. The Nascent Parasitophorous Vacuole Membrane of <i>Encephalitozoon cuniculi</i> Is Formed by Host Cell Lipids and Contains Pores Which Allow Nutrient Uptake

The Nascent Parasitophorous Vacuole Membrane of Encephalitozoon cuniculi Is Formed by Host Cell Lipids and Contains Pores Which Allow Nutrient Uptake

10.1128/ec.00004-08

Crossref journal-article

American Society for Microbiology

Eukaryotic Cell (235)

Abstract

ABSTRACT Microsporidia are obligate intracellular pathogens which enter host cells by the discharge of a hollow tube through which the sporoplasma is extruded into the host cell. Since this invasion mechanism is very different from common entry strategies, the formation of the parasitophorous vacuole (PV) in Encephalitozoon species is likely to be distinct from known principles. We investigated the origin of the nascent Encephalitozoon cuniculi PV membrane with the aid of fluorescent lipid probes. When Bodipy 500/510-C 12 -HPC-labeled spores were used for infection, the emerging PV membrane was unlabeled, suggesting that sporoplasma-derived lipids do not significantly contribute to the formation of the PV membrane. In contrast, when raft and nonraft microdomains of the host cell plasma membrane were selectively labeled with DiIC 16 and Speedy DiO, both tracers were detectable in the nascent PV membrane shortly after infection, indicating that the bulk lipids of the PV membrane are host cell derived. Time-lapse fluorescence microscopy revealed that the formation of the PV membrane is a fast event (<1.3 s), which occurred simultaneously with the extrusion of the sporoplasma. The portion of the discharged tube which is in contact with the host cell was found to be coated with labeled host cell lipids, which might be an indication for a plasma membrane invagination at the contact site. To investigate the presence of pores in the E. cuniculi PV membrane, we microinjected fluorescent dyes of different sizes into infected host cells. A 0.5-kDa dextran as well as 0.8- to 1.1-kDa peptides could rapidly enter the PV, while a 10-kDa dextran was stably excluded from the PV lumen, indicating that the PV membrane possesses pores with an exclusion size of <10 kDa, which should allow metabolite exchange.

Bibliography

RoÌnnebaÌumer, K., Gross, U., & Bohne, W. (2008). The Nascent Parasitophorous Vacuole Membrane of Encephalitozoon cuniculi Is Formed by Host Cell Lipids and Contains Pores Which Allow Nutrient Uptake. Eukaryotic Cell, 7(6), 1001â1008.

Authors 3

Karin Rönnebäumer (first)
Uwe Gross (additional)
Wolfgang Bohne (additional)

References 31 Referenced 34

10.1016/j.ijpara.2007.04.005
Barker, R. J. 1975. Ultrastructural observation on Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923, from mouse peritoneal macrophages. Fol. Parasitol.22:1-9. / Fol. Parasitol. (1975)
Cali, A. 1986. Comparison of the biology of Microsporidia from different host groups, p. 356-359. In R. A. Samson, J. M. Black, and D. Peters (ed.), Fundamental and applied aspects of invertebrate pathology. Foundation for the 4th International Colloquium on Invertebrate Pathology, Wageningen, The Netherlands. / Fundamental and applied aspects of invertebrate pathology. (1986)
Morphogenesis in the genus Nosema. 1971
Cali, A., and P. M. Takvorian. 1999. Developmental morphology and life cycles of the microsporidia, p. 85-128. In M. Wittner and L. M. Weiss (ed.), The microsporidia and microsporidiosis. American Society for Microbiology, Washington, DC. / The microsporidia and microsporidiosis. (1999)
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Vavra, J., and J. I. R. Larsson. 1999. Structure of the microsporidia, p. 7-84. In M. Wittner and L. M. Weiss (ed.). The microsporidia and microsporidiosis. American Society for Microbiology, Washington, DC. / The microsporidia and microsporidiosis. (1999)
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Dates

Type	When
Created	17 years, 4 months ago (April 11, 2008, 8:58 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 9:36 a.m.)
Indexed	4 months, 1 week ago (April 25, 2025, 4:24 a.m.)
Issued	17 years, 3 months ago (June 1, 2008)
Published	17 years, 3 months ago (June 1, 2008)
Published Print	17 years, 3 months ago (June 1, 2008)

Funders 0

None

BibTeX

@article{Ro_nneba_umer_2008, title={The Nascent Parasitophorous Vacuole Membrane of Encephalitozoon cuniculi Is Formed by Host Cell Lipids and Contains Pores Which Allow Nutrient Uptake}, volume={7}, ISSN={1535-9786}, url={http://dx.doi.org/10.1128/ec.00004-08}, DOI={10.1128/ec.00004-08}, number={6}, journal={Eukaryotic Cell}, publisher={American Society for Microbiology}, author={Rönnebäumer, Karin and Gross, Uwe and Bohne, Wolfgang}, year={2008}, month=jun, pages={1001–1008} }

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