DOI: 10.1128/aem.72.4.2775-2782.2006. <i>Geobacter lovleyi</i> sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium

Geobacter lovleyi sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium

10.1128/aem.72.4.2775-2782.2006

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT A bacterial isolate, designated strain SZ, was obtained from noncontaminated creek sediment microcosms based on its ability to derive energy from acetate oxidation coupled to tetrachloroethene (PCE)-to- cis -1,2-dichloroethene ( cis -DCE) dechlorination (i.e., chlororespiration). Hydrogen and pyruvate served as alternate electron donors for strain SZ, and the range of electron acceptors included (reduced products are given in brackets) PCE and trichloroethene [ cis -DCE], nitrate [ammonium], fumarate [succinate], Fe(III) [Fe(II)], malate [succinate], Mn(IV) [Mn(II)], U(VI) [U(IV)], and elemental sulfur [sulfide]. PCE and soluble Fe(III) (as ferric citrate) were reduced at rates of 56.5 and 164 nmol min −1 mg of protein −1 , respectively, with acetate as the electron donor. Alternate electron acceptors, such as U(VI) and nitrate, did not inhibit PCE dechlorination and were consumed concomitantly. With PCE, Fe(III) (as ferric citrate), and nitrate as electron acceptors, H 2 was consumed to threshold concentrations of 0.08 ± 0.03 nM, 0.16 ± 0.07 nM, and 0.5 ± 0.06 nM, respectively, and acetate was consumed to 3.0 ± 2.1 nM, 1.2 ± 0.5 nM, and 3.6 ± 0.25 nM, respectively. Apparently, electron acceptor-specific acetate consumption threshold concentrations exist, suggesting that similar to the hydrogen threshold model, the measurement of acetate threshold concentrations offers an additional diagnostic tool to delineate terminal electron-accepting processes in anaerobic subsurface environments. Genetic and phenotypic analyses classify strain SZ as the type strain of the new species, Geobacter lovleyi sp. nov., with Geobacter (formerly Trichlorobacter ) thiogenes as the closest relative. Furthermore, the analysis of 16S rRNA gene sequences recovered from PCE-dechlorinating consortia and chloroethene-contaminated subsurface environments suggests that Geobacter lovleyi belongs to a distinct, dechlorinating clade within the metal-reducing Geobacter group. Substrate versatility, consumption of electron donors to low threshold concentrations, and simultaneous reduction of electron acceptors suggest that strain SZ-type organisms have desirable characteristics for bioremediation applications.

Bibliography

Sung, Y., Fletcher, K. E., Ritalahti, K. M., Apkarian, R. P., Ramos-HernaÌndez, N., Sanford, R. A., Mesbah, N. M., & LoÌffler, F. E. (2006). Geobacter lovleyi sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium. Applied and Environmental Microbiology, 72(4), 2775â2782.

Authors 8

Youlboong Sung (first)
Kelly E. Fletcher (additional)
Kirsti M. Ritalahti (additional)
Robert P. Apkarian (additional)
Natalia Ramos-Hernández (additional)
Robert A. Sanford (additional)
Noha M. Mesbah (additional)
Frank E. Löffler (additional)

References 53 Referenced 291

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Dates

Type	When
Created	19 years, 5 months ago (April 6, 2006, 9:43 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 11:14 a.m.)
Indexed	5 hours, 54 minutes ago (Sept. 6, 2025, 4:19 p.m.)
Issued	19 years, 5 months ago (April 1, 2006)
Published	19 years, 5 months ago (April 1, 2006)
Published Print	19 years, 5 months ago (April 1, 2006)

Funders 0

None

BibTeX

@article{Sung_2006, title={Geobacter lovleyi sp. nov. Strain SZ, a Novel Metal-Reducing and Tetrachloroethene-Dechlorinating Bacterium}, volume={72}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.72.4.2775-2782.2006}, DOI={10.1128/aem.72.4.2775-2782.2006}, number={4}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Sung, Youlboong and Fletcher, Kelly E. and Ritalahti, Kirsti M. and Apkarian, Robert P. and Ramos-Hernández, Natalia and Sanford, Robert A. and Mesbah, Noha M. and Löffler, Frank E.}, year={2006}, month=apr, pages={2775–2782} }

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