DOI: 10.1128/aem.70.11.6914-6919.2004. Complete Type III Secretion System of a Mesophilic <i>Aeromonas hydrophila</i> Strain

Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain

10.1128/aem.70.11.6914-6919.2004

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa . Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila , A. veronii , and A. caviae . By using one of the TTSS genes ( ascV ), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.

Bibliography

Vilches, S., Urgell, C., Merino, S., ChacÃ³n, M. R., Soler, L., Castro-Escarpulli, G., Figueras, M. J., & TomÃ¡s, J. M. (2004). Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain. Applied and Environmental Microbiology, 70(11), 6914â6919.

Authors 8

Silvia Vilches (first)
Cecilia Urgell (additional)
Susana Merino (additional)
Matilde R. Chacón (additional)
Lara Soler (additional)
Graciela Castro-Escarpulli (additional)
Maria Jose Figueras (additional)
Juan M. Tomás (additional)

References 23 Referenced 77

10.1128/JB.184.21.5966-5970.2002
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10.1128/jb.170.2.499-506.1988
Figueras, M. J., J. Guarro, and A. Martinez-Murcia. 2000. Clinically relevant Aeromonas species. Clin. Infect. Dis.30:988-989. / Clin. Infect. Dis. (2000)
10.1099/00207713-50-6-2069
Howard S. P. S. Macintyre and J. T. Buckley. 1996. The genus Aeromonas p. 267-286. In B. Austin M. Altwegg P. J. Gosling and S. Joseph (ed.) Toxin. John Wiley and Sons Singapore Singapore.
10.1128/JB.185.24.7213-7221.2003
10.1086/514652
Janda J. M. 2001. Aeromonas and Plesiomonas p. 1237-1270. In M. Sussman (ed.) Molecular medical microbiology. Academic Press San Diego Calif. (10.1016/B978-012677530-3/50278-6)
Joseph, S. W., and A. M. Carnahan. 2000. Update on the genus Aeromonas. ASM News66:218-233. / ASM News (2000)
10.1128/iai.56.10.2639-2644.1988
Merino, S., S. Camprubí, and J. M. Tomás. 1993. Incidence of Aeromonas sp. serotypes O:34 and O:11 among clinical isolates. Med. Microbiol. Lett.2:48-55. / Med. Microbiol. Lett. (1993)
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Pemberton, J. M., S. P. Kidd, and R. Schmidt. 1977. Secreted enzymes of Aeromonas. FEMS Microbiol. Lett.152:1-10. / FEMS Microbiol. Lett. (1977)
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Dates

Type	When
Created	20 years, 10 months ago (Nov. 4, 2004, 4:58 p.m.)
Deposited	3 years, 6 months ago (Feb. 22, 2022, 8:52 p.m.)
Indexed	2 months ago (July 2, 2025, 11:39 a.m.)
Issued	20 years, 10 months ago (Nov. 1, 2004)
Published	20 years, 10 months ago (Nov. 1, 2004)
Published Print	20 years, 10 months ago (Nov. 1, 2004)

Funders 0

None

BibTeX

@article{Vilches_2004, title={Complete Type III Secretion System of a Mesophilic Aeromonas hydrophila Strain}, volume={70}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.70.11.6914-6919.2004}, DOI={10.1128/aem.70.11.6914-6919.2004}, number={11}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Vilches, Silvia and Urgell, Cecilia and Merino, Susana and Chacón, Matilde R. and Soler, Lara and Castro-Escarpulli, Graciela and Figueras, Maria Jose and Tomás, Juan M.}, year={2004}, month=nov, pages={6914–6919} }

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