DOI: 10.1128/aem.69.8.4837-4845.2003. The Biphenyl- and 4-Chlorobiphenyl-Catabolic Transposon Tn<i>4371</i>, a Member of a New Family of Genomic Islands Related to IncP and Ti Plasmids

The Biphenyl- and 4-Chlorobiphenyl-Catabolic Transposon Tn4371, a Member of a New Family of Genomic Islands Related to IncP and Ti Plasmids

10.1128/aem.69.8.4837-4845.2003

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACTThe nucleotide sequence of the biphenyl catabolic transposon Tn4371has been completed and analyzed. It confirmed that the element has a mosaic structure made of several building blocks. In addition to previously identified genes coding for a tyrosine recombinase related to phage integrases and for biphenyl degradation enzymes very similar to those ofAchromobacter georgiopolitanumKKS102, Tn4371carries many plasmid-related genes involved in replication, partition, and other, as-yet-unknown, plasmid functions. One gene cluster contains most of the genes required to express a type IV secretion-mating pair formation apparatus coupled with a TraG ATPase, all of which are related to those found on IncP and Ti plasmids. Orthologues of all Tn4371plasmid-related genes and of the tyrosine recombinase gene were found, with a very similar organization, in the chromosome ofRalstonia solanacearumand on the yet-to-be-determined genomic sequences ofErwinia chrysanthemiandAzotobacter vinelandii. In each of these chromosomal segments, conserved segments were separated by different groups of genes, which also differed from the Tn4371 bphgenes. The conserved blocks of genes were also identified, in at least two copies, in the chromosome ofRalstonia metalliduransCH34. Tn4371thus appears to represent a new family of potentially mobile genomic islands with a broad host range since they reside in a wide range of soil proteobacteria, including plant pathogens.

Bibliography

Toussaint, A., Merlin, C., Monchy, S., Benotmane, M. A., Leplae, R., Mergeay, M., & Springael, D. (2003). The Biphenyl- and 4-Chlorobiphenyl-Catabolic Transposon Tn4371, a Member of a New Family of Genomic Islands Related to IncP and Ti Plasmids. Applied and Environmental Microbiology, 69(8), 4837â4845.

Authors 7

Ariane Toussaint (first)
Christophe Merlin (additional)
Sébastien Monchy (additional)
M. Abderrafi Benotmane (additional)
Raphaël Leplae (additional)
Max Mergeay (additional)
Dirk Springael (additional)

References 38 Referenced 89

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Dates

Type	When
Created	22 years, 1 month ago (Aug. 5, 2003, 6:09 p.m.)
Deposited	1 year, 7 months ago (Jan. 10, 2024, 6:35 a.m.)
Indexed	2 months ago (July 1, 2025, 2:26 p.m.)
Issued	22 years, 1 month ago (Aug. 1, 2003)
Published	22 years, 1 month ago (Aug. 1, 2003)
Published Print	22 years, 1 month ago (Aug. 1, 2003)

Funders 0

None

BibTeX

@article{Toussaint_2003, title={The Biphenyl- and 4-Chlorobiphenyl-Catabolic Transposon Tn4371, a Member of a New Family of Genomic Islands Related to IncP and Ti Plasmids}, volume={69}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.69.8.4837-4845.2003}, DOI={10.1128/aem.69.8.4837-4845.2003}, number={8}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Toussaint, Ariane and Merlin, Christophe and Monchy, Sébastien and Benotmane, M. Abderrafi and Leplae, Raphaël and Mergeay, Max and Springael, Dirk}, year={2003}, month=aug, pages={4837–4845} }

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