DOI: 10.1128/aem.68.8.3780-3789.2002. Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in <i>Photorhabdus luminescens</i>

Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in Photorhabdus luminescens

10.1128/aem.68.8.3780-3789.2002

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT The luminescent entomopathogenic bacterium Photorhabdus luminescens produces several yet-uncharacterized broad-spectrum antibiotics. We report the identification and characterization of a cluster of eight genes (named cpmA to cpmH ) responsible for the production of a carbapenem-like antibiotic in strain TT01 of P. luminescens . The cpm cluster differs in several crucial aspects from other car operons. The level of cpm mRNA peaks during exponential phase and is regulated by a Rap/Hor homolog identified in the P. luminescens genome. Marker-exchange mutagenesis of this gene in the entomopathogen decreased antibiotic production. The luxS -like signaling mechanism of quorum sensing also plays a role in the regulation of the cpm operon. Indeed, luxS , which is involved in the production of a newly identified autoinducer, is responsible for repression of cpm gene expression at the end of the exponential growth phase. The importance of this carbapenem production in the ecology of P. luminescens is discussed.

Bibliography

Derzelle, S., Duchaud, E., Kunst, F., Danchin, A., & Bertin, P. (2002). Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in Photorhabdus luminescens. Applied and Environmental Microbiology, 68(8), 3780â3789.

Authors 5

Sylviane Derzelle (first)
Eric Duchaud (additional)
Frank Kunst (additional)
Antoine Danchin (additional)
Philippe Bertin (additional)

References 43 Referenced 112

10.1042/bj2880997
10.1111/j.1365-2958.1993.tb01737.x
Boemare, N., A. Givaudan, M. Brehelin, and C. Laumond. 1997. Symbiosis and pathogenicity of nematode-bacterium complexes. Symbiosis22:21-45. / Symbiosis (1997)
10.1016/S0924-8579(98)00094-6
10.7164/antibiotics.41.1231
Bycroft, B. W., C. Maslen, S. J. Box, A. Brown, and J. W. Tyler. 1987. The isolation and characterisation of (3R,5R)- and (3S,5R)-carbapenem-3-carboxylic acid from Serratia and Erwinia species and their putative biosynthetic role. J. Chem. Soc. Chem. Commun.21:1623-1625. / J. Chem. Soc. Chem. Commun. (1987)
10.1128/JB.183.20.5834-5839.2001
10.1128/jb.173.12.3924-3929.1991
Dunlap, P. V. 1999. Quorum regulation of luminescence in Vibrio fischeri. J. Mol. Microbiol. Biotechnol.1:5-12. / J. Mol. Microbiol. Biotechnol. (1999)
10.1128/jb.179.17.5288-5291.1997
10.1016/S0723-2020(99)80001-0
10.1099/00207713-49-4-1645
10.1146/annurev.micro.51.1.47
10.1128/mr.60.1.21-43.1996
10.1098/rspb.1981.0043
10.1007/BF00409093
10.1006/jipa.1999.4900
10.4065/74.4.420
10.1128/JB.182.13.3638-3643.2000
10.1128/aem.61.12.4329-4333.1995
10.1046/j.1365-2958.1999.01196.x
10.1099/13500872-141-3-541
10.1046/j.1365-2958.1997.6001974.x
10.1046/j.1365-2958.1996.00125.x
10.1111/j.1365-2958.1996.tb02500.x
10.7164/antibiotics.35.653
10.1016/0378-1119(93)90611-6
10.1046/j.1365-2958.2000.01740.x
10.1054/drup.2000.0144
10.1128/aem.54.6.1602-1605.1988
10.1146/annurev.genet.32.1.255
10.2165/00003495-200161050-00001
Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
10.1046/j.1365-2958.2001.02532.x
10.1007/BF00436188
10.1073/pnas.95.12.7046
10.1073/pnas.96.4.1639
10.1046/j.1365-2958.1999.01612.x
10.1046/j.1365-2958.1997.5981976.x
10.1046/j.1365-2958.2000.01872.x
Trezzani I. 2001. Thesis. Institut Pasteur Paris France.
10.1128/JB.182.22.6347-6357.2000
10.1016/S0021-9258(18)89118-9

Dates

Type	When
Created	22 years, 11 months ago (Oct. 1, 2002, 3:36 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 8:17 a.m.)
Indexed	1 month, 2 weeks ago (July 20, 2025, 12:21 a.m.)
Issued	23 years, 1 month ago (Aug. 1, 2002)
Published	23 years, 1 month ago (Aug. 1, 2002)
Published Print	23 years, 1 month ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Derzelle_2002, title={Identification, Characterization, and Regulation of a Cluster of Genes Involved in Carbapenem Biosynthesis in Photorhabdus luminescens}, volume={68}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.68.8.3780-3789.2002}, DOI={10.1128/aem.68.8.3780-3789.2002}, number={8}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Derzelle, Sylviane and Duchaud, Eric and Kunst, Frank and Danchin, Antoine and Bertin, Philippe}, year={2002}, month=aug, pages={3780–3789} }

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