DOI: 10.1128/aem.68.6.3076-3084.2002. Crescent Bodies of <i>Parachlamydia acanthamoeba</i> and Its Life Cycle within <i>Acanthamoeba polyphaga</i> : an Electron Micrograph Study

Crescent Bodies of Parachlamydia acanthamoeba and Its Life Cycle within Acanthamoeba polyphaga : an Electron Micrograph Study

10.1128/aem.68.6.3076-3084.2002

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Parachlamydiaceae are endosymbionts of free-living amoeba first identified in 1997. Two developmental stages, elementary and reticulate bodies, were observed; however, their localization and proportions according to culture condition and duration remain unknown. The life cycle of Parachlamydia acanthamoeba within Acanthamoeba polyphaga was studied by transmission electron microscopy of 8-, 36-, and 144-h coculture. Morphometry and quantification were performed using SAMBA software. The elementary body, the predominant stage within the amoebae, was located mainly within their vacuoles. The multiplication of Parachlamydia bacteria by binary fission of reticulate bodies was independently associated with culture in PYG broth (odds ratio [OR] = 4.4; 95% confidence interval [CI], 1.55 to 12.46) and with the presence of reticulate bodies within the amoebae (OR = 2.10; 95% CI, 1.53 to 2.89). A third developmental stage was observed, the crescent body. Its presence outside and inside the amoebae was associated mainly with prolonged incubation time (OR = 3.98; 95% CI, 1.49 to 10.68, and OR = 5.98; 95% CI, 1.75 to 20.4, respectively). Elementary and crescent bodies were released into the extracellular medium within vesicles or after amoebal lysis. For both, phagocytosis was their mode of entry. This electron micrograph study revealed another infective developmental stage, the crescent body, and provided quantitative analysis of the life cycle of P. acanthamoeba within A. polyphaga .

Bibliography

Greub, G., & Raoult, D. (2002). Crescent Bodies of Parachlamydia acanthamoeba and Its Life Cycle within Acanthamoeba polyphaga â¯: an Electron Micrograph Study. Applied and Environmental Microbiology, 68(6), 3076â3084.

Authors 2

Gilbert Greub (first)
Didier Raoult (additional)

References 27 Referenced 116

10.1128/aem.63.1.115-121.1997
10.1128/AEM.64.1.279-286.1998
10.1099/13500872-142-12-3525
10.1016/S0140-6736(05)62701-8
10.1128/AEM.66.3.1057-1061.2000
10.1128/aem.63.4.1396-1399.1997
10.1128/AEM.66.6.2613-2619.2000
Emerging Infect. Dis.
Harb, O. S., and J. A. Kwaik. 2000. Interaction of Legionella pneumophila with protozoa provides lessons. ASM News66:609-616. / ASM News (2000)
10.1111/j.1365-2672.1995.tb01674.x
10.1099/00221287-146-5-1231
10.1017/S0022172400070248
10.1111/j.1574-6941.2000.tb00762.x
10.1099/00222615-33-1-51
10.1016/S0932-4739(11)80203-8
Michel, R., K. D. Muller, and E. N. Schmid. 1995. Ehrlichia-like organisms (KSL1) observed as obligate intracellular parasites of Saccamoeba species. Endocytobiosis Cell Res.11:69-80. / Endocytobiosis Cell Res. (1995)
Moulder, J. W. 1984. Order II. Chlamydiales, p. 729-739. In N. R. Krieg and J. G. Holt (ed.), Bergey's manual of systematic bacteriology. Baltimore, Md. / Bergey's manual of systematic bacteriology (1984)
Robinson, E. N. Jr., and Z. McGee. 1989. Chlamydiae and a common sexually transmitted disease, p. 330-337. In M. Schaechter, G. Medoff, and D. Schlessinger. (ed.), Mechanisms of microbial disease. Williams & Wilkins, Baltimore, Md. / Mechanisms of microbial disease (1989)
10.3109/10408419409114556
10.1128/AEM.64.5.1822-1824.1998
10.1078/S1438-4639(04)70013-9
10.1136/jcp.33.12.1179
10.1136/jcp.36.9.978
Rubin, S. J. 1987. Chlamydiae, p. 835-842. In B. J. Howard, J. Klass, A. S. Weissfeld, S. J. Rubin, and R. C. Tilton (ed.), Clinical and pathogenic microbiology. The C.V. Mosby Company, Washington, D.C. / Clinical and pathogenic microbiology (1987)
10.1128/AEM.64.6.2256-2261.1998
10.1128/iai.19.3.1054-1060.1978
10.1128/AAC.39.7.1596

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6:09 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 8:02 a.m.)
Indexed	3 months, 4 weeks ago (April 28, 2025, 11:28 a.m.)
Issued	23 years, 2 months ago (June 1, 2002)
Published	23 years, 2 months ago (June 1, 2002)
Published Print	23 years, 2 months ago (June 1, 2002)

Funders 0

None

BibTeX

@article{Greub_2002, title={Crescent Bodies of Parachlamydia acanthamoeba and Its Life Cycle within Acanthamoeba polyphaga  : an Electron Micrograph Study}, volume={68}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.68.6.3076-3084.2002}, DOI={10.1128/aem.68.6.3076-3084.2002}, number={6}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Greub, Gilbert and Raoult, Didier}, year={2002}, month=jun, pages={3076–3084} }

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