DOI: 10.1128/aem.67.5.2208-2212.2001. New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes

New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes

10.1128/aem.67.5.2208-2212.2001

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Attempts have been made to use manganese peroxidase (MnP) for chlorine-free pulp biobleaching, but they have not been commercially viable because of the enzyme's low stability. We developed a new pulp biobleaching method involving mesoporous material-immobilized manganese peroxidase from Phanerochaete chrysosporium . MnP immobilized in FSM-16, a folded-sheet mesoporous material whose pore size is nearly the same as the diameter of the enzyme, had the highest thermal stability and tolerance to H 2 O 2 . MnP immobilized in FSM-16 retained more than 80% of its initial activity even after 10 days of continuous reaction. We constructed a thermally discontinuous two-stage reactor system, in which the enzyme (39°C) and pulp-bleaching (70°C) reactions were performed separately. When the treatment of pulp with MnP by means of the two-stage reactor system and alkaline extraction was repeated seven times, the brightness of the pulp increased to about 88% within 7 h after completion of the last treatment.

Bibliography

Sasaki, T., Kajino, T., Li, B., Sugiyama, H., & Takahashi, H. (2001). New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes. Applied and Environmental Microbiology, 67(5), 2208â2212.

Authors 5

Toshiya Sasaki (first)
Tsutomu Kajino (additional)
Bo Li (additional)
Hidehiko Sugiyama (additional)
Haruo Takahashi (additional)

References 22 Referenced 49

Arbeloa M. Leclerc J. Goma G. Pommier J. C. An evaluation of the potential of lignin peroxidases to improve pulp. TAPPI (Tech. Assoc. Pulp Pap. Ind.) J. 74 1992 123 127 / TAPPI (Tech. Assoc. Pulp Pap. Ind.) J. / An evaluation of the potential of lignin peroxidases to improve pulp. by Arbeloa M. (1992)
10.1021/ja00053a020
10.1007/BF00172202
10.3109/07388558709086984
10.1128/AEM.64.4.1366-1371.1998
10.1016/0003-9861(86)90378-4
10.1021/bk-1989-0389.ch009
10.1016/0003-9861(84)90280-7
10.1002/(SICI)1097-0290(19981020)60:2<204::AID-BIT8>3.0.CO;2-R
10.1007/BF02788055
10.1016/0003-9861(89)90044-1
10.1246/bcsj.69.1449
10.1146/annurev.mi.41.100187.002341
10.1016/0141-0229(86)90006-2
10.1128/aem.60.12.4359-4363.1994
10.1128/aem.60.3.921-926.1994
10.2134/jeq1991.00472425002000030002x
10.1016/0003-9861(91)90176-J
10.1021/cm000487a
10.1046/j.1432-1033.2000.01469.x
Wiseman A. Potential for immobilized enzymes in water and air purification. Top. Enzyme Ferment. Biotechnol. 7 1983 264 270 / Top. Enzyme Ferment. Biotechnol. / Potential for immobilized enzymes in water and air purification. by Wiseman A. (1983)
10.1038/24132

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 6 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 6:37 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 5, 2025, 9:08 a.m.)
Issued	24 years, 4 months ago (May 1, 2001)
Published	24 years, 4 months ago (May 1, 2001)
Published Print	24 years, 4 months ago (May 1, 2001)

Funders 0

None

BibTeX

@article{Sasaki_2001, title={New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes}, volume={67}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.67.5.2208-2212.2001}, DOI={10.1128/aem.67.5.2208-2212.2001}, number={5}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Sasaki, Toshiya and Kajino, Tsutomu and Li, Bo and Sugiyama, Hidehiko and Takahashi, Haruo}, year={2001}, month=may, pages={2208–2212} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T13:08:15Z",
    "timestamp": 1754399295041
  },
  "reference-count": 22,
  "publisher": "American Society for Microbiology",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            5,
            1
          ]
        ],
        "date-time": "2001-05-01T00:00:00Z",
        "timestamp": 988675200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Attempts have been made to use manganese peroxidase (MnP) for chlorine-free pulp biobleaching, but they have not been commercially viable because of the enzyme's low stability. We developed a new pulp biobleaching method involving mesoporous material-immobilized manganese peroxidase from\n            <jats:italic>Phanerochaete chrysosporium</jats:italic>\n            . MnP immobilized in FSM-16, a folded-sheet mesoporous material whose pore size is nearly the same as the diameter of the enzyme, had the highest thermal stability and tolerance to H\n            <jats:sub>2</jats:sub>\n            O\n            <jats:sub>2</jats:sub>\n            . MnP immobilized in FSM-16 retained more than 80% of its initial activity even after 10 days of continuous reaction. We constructed a thermally discontinuous two-stage reactor system, in which the enzyme (39\u00b0C) and pulp-bleaching (70\u00b0C) reactions were performed separately. When the treatment of pulp with MnP by means of the two-stage reactor system and alkaline extraction was repeated seven times, the brightness of the pulp increased to about 88% within 7 h after completion of the last treatment.\n          </jats:p>",
  "DOI": "10.1128/aem.67.5.2208-2212.2001",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        27
      ]
    ],
    "date-time": "2002-07-27T10:00:58Z",
    "timestamp": 1027764058000
  },
  "page": "2208-2212",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 49,
  "title": "New Pulp Biobleaching System Involving Manganese Peroxidase Immobilized in a Silica Support with Controlled Pore Sizes",
  "prefix": "10.1128",
  "volume": "67",
  "author": [
    {
      "given": "Toshiya",
      "family": "Sasaki",
      "sequence": "first",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Toyota Central R&amp;D Laboratories, Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192, Japan"
        }
      ]
    },
    {
      "given": "Tsutomu",
      "family": "Kajino",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Toyota Central R&amp;D Laboratories, Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192, Japan"
        }
      ]
    },
    {
      "given": "Bo",
      "family": "Li",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Toyota Central R&amp;D Laboratories, Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192, Japan"
        }
      ]
    },
    {
      "given": "Hidehiko",
      "family": "Sugiyama",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Toyota Central R&amp;D Laboratories, Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192, Japan"
        }
      ]
    },
    {
      "given": "Haruo",
      "family": "Takahashi",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "<!--label omitted: 1-->Toyota Central R&amp;D Laboratories, Inc., 41-1, Yokomichi, Nagakute, Aichi 480-1192, Japan"
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "e_1_3_2_2_2",
      "first-page": "123",
      "article-title": "An evaluation of the potential of lignin peroxidases to improve pulp.",
      "volume": "74",
      "author": "Arbeloa M.",
      "year": "1992",
      "unstructured": "Arbeloa M. Leclerc J. Goma G. Pommier J. C. An evaluation of the potential of lignin peroxidases to improve pulp. TAPPI (Tech. Assoc. Pulp Pap. Ind.) J. 74 1992 123 127",
      "journal-title": "TAPPI (Tech. Assoc. Pulp Pap. Ind.) J."
    },
    {
      "key": "e_1_3_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja00053a020"
    },
    {
      "key": "e_1_3_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00172202"
    },
    {
      "key": "e_1_3_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3109/07388558709086984"
    },
    {
      "key": "e_1_3_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/AEM.64.4.1366-1371.1998"
    },
    {
      "key": "e_1_3_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(86)90378-4"
    },
    {
      "key": "e_1_3_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bk-1989-0389.ch009"
    },
    {
      "key": "e_1_3_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(84)90280-7"
    },
    {
      "key": "e_1_3_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0290(19981020)60:2<204::AID-BIT8>3.0.CO;2-R"
    },
    {
      "key": "e_1_3_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02788055"
    },
    {
      "key": "e_1_3_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(89)90044-1"
    },
    {
      "key": "e_1_3_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1246/bcsj.69.1449"
    },
    {
      "key": "e_1_3_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.mi.41.100187.002341"
    },
    {
      "key": "e_1_3_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0141-0229(86)90006-2"
    },
    {
      "key": "e_1_3_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/aem.60.12.4359-4363.1994"
    },
    {
      "key": "e_1_3_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/aem.60.3.921-926.1994"
    },
    {
      "key": "e_1_3_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.2134/jeq1991.00472425002000030002x"
    },
    {
      "key": "e_1_3_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(91)90176-J"
    },
    {
      "key": "e_1_3_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/cm000487a"
    },
    {
      "key": "e_1_3_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1432-1033.2000.01469.x"
    },
    {
      "key": "e_1_3_2_22_2",
      "first-page": "264",
      "article-title": "Potential for immobilized enzymes in water and air purification.",
      "volume": "7",
      "author": "Wiseman A.",
      "year": "1983",
      "unstructured": "Wiseman A. Potential for immobilized enzymes in water and air purification. Top. Enzyme Ferment. Biotechnol. 7 1983 264 270",
      "journal-title": "Top. Enzyme Ferment. Biotechnol."
    },
    {
      "key": "e_1_3_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/24132"
    }
  ],
  "container-title": "Applied and Environmental Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/AEM.67.5.2208-2212.2001",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/AEM.67.5.2208-2212.2001",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        2,
        23
      ]
    ],
    "date-time": "2022-02-23T11:37:07Z",
    "timestamp": 1645616227000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/AEM.67.5.2208-2212.2001"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2001,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/AEM.67.5.2208-2212.2001"
  ],
  "URL": "http://dx.doi.org/10.1128/AEM.67.5.2208-2212.2001",
  "relation": {},
  "ISSN": [
    "0099-2240",
    "1098-5336"
  ],
  "subject": [],
  "container-title-short": "Appl Environ Microbiol",
  "published": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "2000-11-21",
      "order": 0,
      "name": "received",
      "label": "Received",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2001-03-06",
      "order": 1,
      "name": "accepted",
      "label": "Accepted",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    },
    {
      "value": "2001-05-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}