DOI: 10.1128/aem.66.8.3221-3229.2000. Purification and Biochemical Characterization of Mutacin I from the Group I Strain of <i>Streptococcus mutans</i> , CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes

Purification and Biochemical Characterization of Mutacin I from the Group I Strain of Streptococcus mutans , CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes

10.1128/aem.66.8.3221-3229.2000

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Previously, we reported isolation and characterization of mutacin III and genetic analysis of mutacin III biosynthesis genes from the group III strain of Streptococcus mutans , UA787 (F. Qi, P. Chen, and P. W. Caufield, Appl. Environ. Microbiol. 65:3880–3887, 1999). During the same process of isolating the mutacin III structural gene, we also cloned the structural gene for mutacin I. In this report, we present purification and biochemical characterization of mutacin I from the group I strain CH43 and compare mutacin I and mutacin III biosynthesis genes. The mutacin I biosynthesis gene locus consists of 14 genes in the order mutR , - A , - A ′, - B , - C , - D , - P , - T , - F , - E , - G , orfX , orfY , orfZ. mutA is the structural gene for mutacin I, while mutA ′ is not required for mutacin I activity. DNA and protein sequence analysis revealed that mutacins I and III are homologous to each other, possibly arising from a common ancestor. The mature mutacin I is 24 amino acids in size and has a molecular mass of 2,364 Da. Ethanethiol modification and peptide sequencing of mutacin I revealed that it contains six dehydrated serines, four of which are probably involved with thioether bridge formation. Comparison of the primary sequence of mutacin I with that of mutacin III and epidermin suggests that mutacin I likely has the same bridging pattern as epidermin.

Bibliography

Qi, F., Chen, P., & Caufield, P. W. (2000). Purification and Biochemical Characterization of Mutacin I from the Group I Strain of Streptococcus mutans , CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes. Applied and Environmental Microbiology, 66(8), 3221â3229.

Authors 3

Fengxia Qi (first)
Ping Chen (additional)
Page W. Caufield (additional)

References 38 Referenced 80

10.1111/j.1432-1033.1986.tb09933.x
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10.1128/AEM.65.3.1356-1360.1999
Creighton T. G. Proteins: structures and molecular principles 1983 235 W. H. Freeman and Company New York N.Y
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 5:19 a.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 5:45 p.m.)
Issued	25 years, 1 month ago (Aug. 1, 2000)
Published	25 years, 1 month ago (Aug. 1, 2000)
Published Print	25 years, 1 month ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Qi_2000, title={Purification and Biochemical Characterization of Mutacin I from the Group I Strain of Streptococcus mutans , CH43, and Genetic Analysis of Mutacin I Biosynthesis Genes}, volume={66}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.66.8.3221-3229.2000}, DOI={10.1128/aem.66.8.3221-3229.2000}, number={8}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Qi, Fengxia and Chen, Ping and Caufield, Page W.}, year={2000}, month=aug, pages={3221–3229} }

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