DOI: 10.1128/aem.66.5.1970-1973.2000. Redirection of the Respiro-Fermentative Flux Distribution in <i>Saccharomyces cerevisiae</i> by Overexpression of the Transcription Factor Hap4p

Redirection of the Respiro-Fermentative Flux Distribution in Saccharomyces cerevisiae by Overexpression of the Transcription Factor Hap4p

10.1128/aem.66.5.1970-1973.2000

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae . Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms.

Bibliography

Blom, J., De Mattos, M. J. T., & Grivell, L. A. (2000). Redirection of the Respiro-Fermentative Flux Distribution in Saccharomyces cerevisiae by Overexpression of the Transcription Factor Hap4p. Applied and Environmental Microbiology, 66(5), 1970â1973.

Authors 3

Jolanda Blom (first)
M. Joost Teixeira De Mattos (additional)
Leslie A. Grivell (additional)

References 32 Referenced 76

10.1038/4462
10.1042/bj2980367
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Fondrat C. Kalogeropoulos A. Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: application to chromosome III. Comput. Appl. Biosci. 12 1996 363 374 / Comput. Appl. Biosci. / Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: application to chromosome III. by Fondrat C. (1996)
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Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 5:22 a.m.)
Indexed	1 year ago (Aug. 27, 2024, 1:05 p.m.)
Issued	25 years, 4 months ago (May 1, 2000)
Published	25 years, 4 months ago (May 1, 2000)
Published Print	25 years, 4 months ago (May 1, 2000)

Funders 0

None

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