DOI: 10.1128/aem.66.3.1026-1030.2000. pH Regulation of Pectate Lyase Secretion Modulates the Attack of<i>Colletotrichum gloeosporioides</i>on Avocado Fruits

pH Regulation of Pectate Lyase Secretion Modulates the Attack ofColletotrichum gloeosporioideson Avocado Fruits

10.1128/aem.66.3.1026-1030.2000

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACTGrowth ofColletotrichum gloeosporioidesin pectolytic enzyme-inducing medium (PEIM) increased the pH of the medium from 3.8 to 6.5. Pectate lyase (PL) secretion was detected when the pH reached 5.8, and the level of secretion increased up to pH 6.5. PL gene (pel) transcript production began at pH 5.0 and increased up to pH 5.7. PL secretion was never detected when the pH of the inducing medium was lower than 5.8 or whenC. gloeosporioideshyphae were transferred from PL-secreting conditions at pH 6.5 to pH 3.8. This behavior differed from that of polygalacturonase (PG), wherepgtranscripts and protein secretion were detected at pH 5.0 and continued up to 5.7. Under in vivo conditions, the pH of unripe pericarp of freshly harvested avocado (Persea americanacv. Fuerte) fruits, resistant toC. gloeosporioidesattack, was 5.2, whereas in ripe fruits, when decay symptoms were expressed, the pericarp pH had increased to 6.3. Two avocado cultivars, Ardit and Ettinger, which are resistant toC. gloeosporioidesattack, had pericarp pHs of less than 5.5, which did not increase during ripening. The present results suggest that host pH regulates the secretion of PL and may affectC. gloeosporioidespathogenicity. The mechanism found in avocado may have equivalents in other postharvest pathosystems and suggests new approaches for breeding against and controlling postharvest diseases.

Bibliography

Yakoby, N., Kobiler, I., Dinoor, A., & Prusky, D. (2000). pH Regulation of Pectate Lyase Secretion Modulates the Attack ofColletotrichum gloeosporioideson Avocado Fruits. Applied and Environmental Microbiology, 66(3), 1026â1030.

Authors 4

Nir Yakoby (first)
Ilana Kobiler (additional)
Amos Dinoor (additional)
Dov Prusky (additional)

References 28 Referenced 110

10.1023/A:1008656013255
10.1007/BF00294682
10.1094/MPMI.1997.10.6.769
10.1094/MPMI-8-0632
Dean A. R. Timberlake E. W. Regulation of the Aspergillus nidulans pectate lyase gene (pelA). Plant Cell 1 1989 275 284 / Plant Cell / Regulation of the Aspergillus nidulans pectate lyase gene (pelA). by Dean A. R. (1989)
10.1002/j.1460-2075.1993.tb06072.x
10.1094/PHYTO.1997.87.6.643
10.1006/pmpp.1993.1061
10.1006/fgbi.1998.1087
10.1016/0925-5214(92)90034-M
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10.1099/00221287-139-10-2295
10.1128/MCB.17.10.5960
10.1101/gad.9.13.1622
Ortega J. Pectolytic enzymes produced by the phytopathogenic fungus Colletotrichum gloeosporioides. Tex. J. Sci. 48 1996 123 128 / Tex. J. Sci. / Pectolytic enzymes produced by the phytopathogenic fungus Colletotrichum gloeosporioides. by Ortega J. (1996)
10.1007/s004380050233
10.1146/annurev.phyto.34.1.413
10.1094/PD-77-0114
10.1111/j.1439-0434.1988.tb04461.x
10.1016/0885-5765(89)90082-9
10.1111/j.1365-3059.1991.tb02291.x
Sambrook J. Fritsch F. E. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
Simmonds J. H. Latent infection in tropical fruits discussed in relation to part played by species of Gloeosporium and Colletotrichum. Proc. R. Soc. Queensl. 52 1941 92 120 (10.5962/p.351669) / Proc. R. Soc. Queensl. / Latent infection in tropical fruits discussed in relation to part played by species of Gloeosporium and Colletotrichum. by Simmonds J. H. (1941)
10.1128/AEM.64.2.709-713.1998
Tu J. C. An improved Mathur's medium for growth, sporulation and germination of spores of Colletotrichum lindemuthianum. Microbiosis 44 1985 87 93 / Microbiosis / An improved Mathur's medium for growth, sporulation and germination of spores of Colletotrichum lindemuthianum. by Tu J. C. (1985)
10.1094/MPMI-7-0293
10.1094/MPMI-8-0621
10.1006/pmpp.1997.0080

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:55 a.m.)
Deposited	2 years, 4 months ago (April 23, 2023, 10:51 a.m.)
Indexed	1 year, 1 month ago (July 9, 2024, 6:31 p.m.)
Issued	25 years, 5 months ago (March 1, 2000)
Published	25 years, 5 months ago (March 1, 2000)
Published Print	25 years, 5 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Yakoby_2000, title={pH Regulation of Pectate Lyase Secretion Modulates the Attack ofColletotrichum gloeosporioideson Avocado Fruits}, volume={66}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.66.3.1026-1030.2000}, DOI={10.1128/aem.66.3.1026-1030.2000}, number={3}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Yakoby, Nir and Kobiler, Ilana and Dinoor, Amos and Prusky, Dov}, year={2000}, month=mar, pages={1026–1030} }

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