DOI: 10.1128/aem.65.9.4134-4140.1999. Staphylococcal Surface Display of Immunoglobulin A (IgA)- and IgE-Specific In Vitro-Selected Binding Proteins (Affibodies) Based on <i>Staphylococcus aureus</i> Protein A

Staphylococcal Surface Display of Immunoglobulin A (IgA)- and IgE-Specific In Vitro-Selected Binding Proteins (Affibodies) Based on Staphylococcus aureus Protein A

10.1128/aem.65.9.4134-4140.1999

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT An expression system designed for cell surface display of hybrid proteins on Staphylococcus carnosus has been evaluated for the display of Staphylococcus aureus protein A (SpA) domains, normally binding to immunoglobulin G (IgG) Fc but here engineered by combinatorial protein chemistry to yield SpA domains, denoted affibodies, with new binding specificities. Such affibodies, with human IgA or IgE binding activity, have previously been selected from a phage library, based on an SpA domain. In this study, these affibodies have been genetically introduced in monomeric or dimeric forms into chimeric proteins expressed on the surface of S. carnosus by using translocation signals from a Staphylococcus hyicus lipase construct together with surface-anchoring regions of SpA. The recombinant surface proteins, containing the IgA- or IgE-specific affibodies, were demonstrated to be expressed as full-length proteins, localized and properly exposed at the cell surface of S. carnosus . Furthermore, these chimeric receptors were found to be functional, since recombinant S. carnosus cells were shown to have gained IgA and IgE binding capacity, respectively. In addition, a positive effect in terms of IgA and IgE reactivity was observed when dimeric versions of the affibodies were present. Potential applications for recombinant bacteria with redirected binding specificity in their surface proteins are discussed.

Bibliography

Gunneriusson, E., Samuelson, P., Ringdahl, J., GroÌnlund, H., Nygren, P.-â«ke, & StÃ¥hl, S. (1999). Staphylococcal Surface Display of Immunoglobulin A (IgA)- and IgE-Specific In Vitro-Selected Binding Proteins (Affibodies) Based on Staphylococcus aureus Protein A. Applied and Environmental Microbiology, 65(9), 4134â4140.

Authors 6

Elin Gunneriusson (first)
Patrik Samuelson (additional)
Jenny Ringdahl (additional)
Hans Grönlund (additional)
Per-Åke Nygren (additional)
Stefan Ståhl (additional)

References 48 Referenced 43

Acheson D. W. K. Sonenshein A. L. Leong J. M. Keusch G. T. Heat-stable spore-based vaccines: surface expression of invasin-cell wall fusion proteins in Bacillus subtilis Vaccines 97. Brown F. Burton D. Doherty P. Mekalanos J. Norrby E. 1997 179 184 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
Andréoni C. Goetsch L. Libon C. Samuelson P. Nguyen T. N. Robert A. Uhlén M. Binz H. Ståhl S. Flow cytometric quantification of surface-displayed recombinant receptors on staphylococci. BioTechniques 23 1997 696 704 / BioTechniques / Flow cytometric quantification of surface-displayed recombinant receptors on staphylococci. by Andréoni C. (1997)
10.1128/jb.176.11.3218-3223.1994
Cano F. S. Liljeqvist T. N. Nguyen P. Samuelson J.-Y. Bonnefoy S. Ståhl and A. Robert. A surface-displayed cholera toxin B peptide improves antibody responses using food-grade staphylococci for mucosal subunit vaccine delivery. FEMS Immunol. Med. Microbiol. in press.
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Francisco J. A. Stathopoulos C. Warren R. A. Kilburn D. G. Georgiou G. Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. Bio/Technology 11 1993 491 495 / Bio/Technology / Specific adhesion and hydrolysis of cellulose by intact Escherichia coli expressing surface anchored cellulase or cellulose binding domains. by Francisco J. A. (1993)
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Gunneriusson E. K. Nord M. Uhlén and P.-Å. Nygren. Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling. Protein Eng. in press.
Gunneriusson E. J. Ringdahl M. Högbom H. Grönlund and P.-Å. Nygren. Unpublished data.
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Hammes W. P. Bosch I. Wolf G. Contribution of Staphylococcus carnosus and Staphylococcus piscifermentans to the fermentation of protein foods. J. Appl. Bacteriol. Symp. Suppl. 79 1995 76S 83S / J. Appl. Bacteriol. Symp. Suppl. / Contribution of Staphylococcus carnosus and Staphylococcus piscifermentans to the fermentation of protein foods. by Hammes W. P. (1995)
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Ståhl S. Samuelson P. Hansson M. Andréoni C. Goetsch L. Libon C. Liljeqvist S. Gunneriusson E. Binz H. Nguyen T. N. Uhlén M. Development of non-pathogenic staphylococci as vaccine delivery vehicles Gram-positive bacteria: vaccine vehicles for mucosal immunization. Pozzi G. Wells J. M. 1997 62 81 Landes Bioscience Georgetown Tex (10.1007/978-3-662-07548-7_4)
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Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 2:55 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 4:05 a.m.)
Indexed	3 months, 3 weeks ago (May 13, 2025, 6:47 a.m.)
Issued	26 years ago (Sept. 1, 1999)
Published	26 years ago (Sept. 1, 1999)
Published Print	26 years ago (Sept. 1, 1999)

Funders 0

None

BibTeX

@article{Gunneriusson_1999, title={Staphylococcal Surface Display of Immunoglobulin A (IgA)- and IgE-Specific In Vitro-Selected Binding Proteins (Affibodies) Based on Staphylococcus aureus Protein A}, volume={65}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.65.9.4134-4140.1999}, DOI={10.1128/aem.65.9.4134-4140.1999}, number={9}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Gunneriusson, Elin and Samuelson, Patrik and Ringdahl, Jenny and Grönlund, Hans and Nygren, Per-Åke and Ståhl, Stefan}, year={1999}, month=sep, pages={4134–4140} }

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