DOI: 10.1128/aem.65.10.4346-4350.1999. Cloning and Analysis of the <scp>l</scp> -Lactate Utilization Genes from <i>Streptococcus iniae</i>

Cloning and Analysis of the l -Lactate Utilization Genes from Streptococcus iniae

10.1128/aem.65.10.4346-4350.1999

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT The presence of lactate oxidase was examined in eight Streptococcus species and some related species of bacteria. A clone (pGR002) was isolated from a genomic library of Streptococcus iniae generated in Escherichia coli , containing a DNA fragment spanning two genes designated lctO and lctP . We show that these genes are likely to be involved in the l -lactic acid aerobic metabolism of this organism. This DNA fragment has been sequenced and characterized. A comparison of the deduced amino acid sequence of LctP protein demonstrated that the protein had significant homology with the l -lactate permeases of other bacteria. The amino acid sequence of the LctO protein of S. iniae also showed a strong homology to l -lactate oxidase from Aerococcus viridans and some NAD-independent lactate dehydrogenases, all belonging to the family of flavin mononucleotide-dependent α-hydroxyacid-oxidizing enzymes. Biochemical assays of the gene products confirm the identity of the genes from the isolated DNA fragment and reveal a possible role for the lactate oxidase from S. iniae . This lactate oxidase is discussed in relation to the growth of the organism in response to carbon source availability.

Bibliography

Gibello, A., Collins, M. D., DomiÌnguez, L., FernaÌndez-GarayzaÌbal, J. F., & Richardson, P. T. (1999). Cloning and Analysis of the l -Lactate Utilization Genes from Streptococcus iniae. Applied and Environmental Microbiology, 65(10), 4346â4350.

Authors 5

A. Gibello (first)
M. D. Collins (additional)
L. Domínguez (additional)
J. F. Fernández-Garayzábal (additional)
P. T. Richardson (additional)

References 34 Referenced 35

10.1016/S0723-2020(11)80279-1
10.1016/0378-4347(91)80167-B
10.1016/0003-2697(76)90527-3
Condon S. Aerobic metabolism of lactic acid bacteria. Ir. J. Food Sci. Technol. 7 1983 15 25 / Ir. J. Food Sci. Technol. / Aerobic metabolism of lactic acid bacteria. by Condon S. (1983)
10.1007/s002849900162
10.1128/jb.175.20.6671-6678.1993
10.1016/0003-9969(85)90004-4
10.1016/0006-291X(89)91546-5
10.1128/iai.59.11.3930-3934.1991
10.1016/0076-6879(94)31038-6
10.1126/science.7542800
10.1128/mr.44.1.106-139.1980
10.1016/S0021-9258(19)39195-1
10.1111/j.1432-1033.1977.tb11859.x
10.1016/S0076-6879(82)89065-4
10.1128/iai.64.10.4319-4323.1996
10.1007/BF00399499
10.1128/aem.57.8.2413-2417.1991
10.1111/j.1574-6968.1989.tb03594.x
10.1111/j.1432-1033.1985.tb09213.x
10.1128/jb.174.21.6956-6964.1992
10.1016/0300-9084(96)88178-8
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10.1111/j.1365-2672.1979.tb01762.x
10.1007/BF00127437
10.1093/oxfordjournals.jbchem.a123004
10.1577/1548-8667(1994)006<0335:SIAWMO>2.3.CO;2
10.1073/pnas.74.12.5463
10.1016/S0021-9258(18)47662-4
10.1093/nar/20.suppl.2111
10.1056/NEJM199708283370902
10.1007/s002849900197
10.1111/j.1574-6968.1984.tb00218.x
Zülli F. Weber H. Zuber H. Nucleotide sequences of lactate dehydrogenase genes from the thermophilic bacteria Bacillus stearothermophilus,B. caldolyticus and B. caldotenax. Biol. Chem. 368 1987 1167 1177 / Biol. Chem. / Nucleotide sequences of lactate dehydrogenase genes from the thermophilic bacteria Bacillus stearothermophilus,B. caldolyticus and B. caldotenax. by Zülli F. (1987)

Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 2:42 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 2:01 a.m.)
Indexed	2 days, 5 hours ago (Aug. 26, 2025, 3:06 a.m.)
Issued	25 years, 10 months ago (Oct. 1, 1999)
Published	25 years, 10 months ago (Oct. 1, 1999)
Published Print	25 years, 10 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{Gibello_1999, title={Cloning and Analysis of the <scp>l</scp> -Lactate Utilization Genes from Streptococcus iniae}, volume={65}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.65.10.4346-4350.1999}, DOI={10.1128/aem.65.10.4346-4350.1999}, number={10}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Gibello, A. and Collins, M. D. and Domínguez, L. and Fernández-Garayzábal, J. F. and Richardson, P. T.}, year={1999}, month=oct, pages={4346–4350} }

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