DOI: 10.1128/aem.65.1.175-180.1999. Production of Biogenic Mn Oxides by <i>Leptothrix discophora</i> SS-1 in a Chemically Defined Growth Medium and Evaluation of Their Pb Adsorption Characteristics

Production of Biogenic Mn Oxides by Leptothrix discophora SS-1 in a Chemically Defined Growth Medium and Evaluation of Their Pb Adsorption Characteristics

10.1128/aem.65.1.175-180.1999

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Biogenic Mn oxides were produced by the bacterium Leptothrix discophora SS-1 (= ATCC 3182) in a chemically defined mineral salts medium, and the Pb binding and specific surface area of these oxides were characterized. Growth of SS-1 in the defined medium with pyruvate as a carbon and energy source required the addition of vitamin B 12 . Complete oxidation of Mn(II) within 60 h required the addition of ≥0.1 μM FeSO 4 . Pb adsorption isotherms were determined for the biogenic Mn oxides (and associated cells with their extracellular polymer) and compared to the Pb adsorption isotherms of cells and exopolymer alone, as well as to abiotic Mn oxides. The Pb adsorption to cells and exopolymer with biogenic Mn oxides (0.8 mmol of Mn per g) at pH 6.0 and 25°C was 2 orders of magnitude greater than the Pb adsorption to cells and exopolymer alone (on a dry weight basis). The Pb adsorption to the biogenic Mn oxide was two to five times greater than the Pb adsorption to a chemically precipitated abiotic Mn oxide and several orders of magnitude greater than the Pb adsorption to two commercially available crystalline MnO 2 minerals. The N 2 Brunauer-Emmet-Teller specific surface areas of the biogenic Mn oxide and fresh Mn oxide precipitate (224 and 58 m 2 /g, respectively) were significantly greater than those of the commercial Mn oxide minerals (0.048 and 4.7 m 2 /g). The Pb adsorption capacity of the biogenic Mn oxide also exceeded that of a chemically precipitated colloidal hydrous Fe oxide under similar solution conditions. These results show that amorphous biogenic Mn oxides similar to those produced by SS-1 may play a significant role in the control of trace metal phase distribution in aquatic systems.

Bibliography

Nelson, Y. M., Lion, L. W., Ghiorse, W. C., & Shuler, M. L. (1999). Production of Biogenic Mn Oxides by Leptothrix discophora SS-1 in a Chemically Defined Growth Medium and Evaluation of Their Pb Adsorption Characteristics. Applied and Environmental Microbiology, 65(1), 175â180.

Authors 4

Yarrow M. Nelson (first)
Leonard W. Lion (additional)
William C. Ghiorse (additional)
Michael L. Shuler (additional)

References 66 Referenced 170

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 2:42 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 2:36 a.m.)
Indexed	3 months, 2 weeks ago (May 21, 2025, 11:03 a.m.)
Issued	26 years, 8 months ago (Jan. 1, 1999)
Published	26 years, 8 months ago (Jan. 1, 1999)
Published Print	26 years, 8 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Nelson_1999, title={Production of Biogenic Mn Oxides by Leptothrix discophora SS-1 in a Chemically Defined Growth Medium and Evaluation of Their Pb Adsorption Characteristics}, volume={65}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.65.1.175-180.1999}, DOI={10.1128/aem.65.1.175-180.1999}, number={1}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Nelson, Yarrow M. and Lion, Leonard W. and Ghiorse, William C. and Shuler, Michael L.}, year={1999}, month=jan, pages={175–180} }

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  "abstract": "<jats:title>ABSTRACT</jats:title>\n          <jats:p>\n            Biogenic Mn oxides were produced by the bacterium\n            <jats:italic>Leptothrix discophora</jats:italic>\n            SS-1 (= ATCC 3182) in a chemically defined mineral salts medium, and the Pb binding and specific surface area of these oxides were characterized. Growth of SS-1 in the defined medium with pyruvate as a carbon and energy source required the addition of vitamin B\n            <jats:sub>12</jats:sub>\n            . Complete oxidation of Mn(II) within 60 h required the addition of \u22650.1 \u03bcM FeSO\n            <jats:sub>4</jats:sub>\n            . Pb adsorption isotherms were determined for the biogenic Mn oxides (and associated cells with their extracellular polymer) and compared to the Pb adsorption isotherms of cells and exopolymer alone, as well as to abiotic Mn oxides. The Pb adsorption to cells and exopolymer with biogenic Mn oxides (0.8 mmol of Mn per g) at pH 6.0 and 25\u00b0C was 2 orders of magnitude greater than the Pb adsorption to cells and exopolymer alone (on a dry weight basis). The Pb adsorption to the biogenic Mn oxide was two to five times greater than the Pb adsorption to a chemically precipitated abiotic Mn oxide and several orders of magnitude greater than the Pb adsorption to two commercially available crystalline MnO\n            <jats:sub>2</jats:sub>\n            minerals. The N\n            <jats:sub>2</jats:sub>\n            Brunauer-Emmet-Teller specific surface areas of the biogenic Mn oxide and fresh Mn oxide precipitate (224 and 58 m\n            <jats:sup>2</jats:sup>\n            /g, respectively) were significantly greater than those of the commercial Mn oxide minerals (0.048 and 4.7 m\n            <jats:sup>2</jats:sup>\n            /g). The Pb adsorption capacity of the biogenic Mn oxide also exceeded that of a chemically precipitated colloidal hydrous Fe oxide under similar solution conditions. These results show that amorphous biogenic Mn oxides similar to those produced by SS-1 may play a significant role in the control of trace metal phase distribution in aquatic systems.\n          </jats:p>",
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