DOI: 10.1128/aem.64.5.1620-1627.1998. High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria

High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria

10.1128/aem.64.5.1620-1627.1998

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT Soils samples were obtained from pristine ecosystems in six regions on five continents. Two of the regions were boreal forests, and the other four were Mediterranean ecosystems. Twenty-four soil samples from each of four or five sites in each of the regions were enriched by using 3-chlorobenzoate (3CBA), and 3CBA mineralizers were isolated from most samples. These isolates were analyzed for the ability to mineralize 3CBA, and genotypes were determined with repetitive extragenic palindromic PCR genomic fingerprints and restriction digests of the 16S rRNA genes (amplified ribosomal DNA restriction analysis [ARDRA]). We found that our collection of 150 stable 3CBA-mineralizing isolates included 48 genotypes and 44 ARDRA types, which formed seven distinct clusters. The majority (91%) of the genotypes were unique to the sites from which they were isolated, and each genotype was found only in the region from which it was isolated. A total of 43 of the 44 ARDRA types were found in only one region. A few genotypes were repeatedly found in one region but not in any other continental region, suggesting that they are regionally endemic. A correlation between bacterial genotype and vegetative community was found for the South African samples. These results suggest that the ability to mineralize 3CBA is distributed among very diverse genotypes and that the genotypes are not globally dispersed.

Bibliography

Fulthorpe, R. R., Rhodes, A. N., & Tiedje, J. M. (1998). High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria. Applied and Environmental Microbiology, 64(5), 1620â1627.

Authors 3

R. R. Fulthorpe (first)
A. N. Rhodes (additional)
J. M. Tiedje (additional)

References 36 Referenced 90

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Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 2:34 p.m.)
Deposited	3 years, 6 months ago (Feb. 22, 2022, 8:37 p.m.)
Indexed	2 months, 2 weeks ago (June 4, 2025, 5:47 p.m.)
Issued	27 years, 3 months ago (May 1, 1998)
Published	27 years, 3 months ago (May 1, 1998)
Published Print	27 years, 3 months ago (May 1, 1998)

Funders 0

None

BibTeX

@article{Fulthorpe_1998, title={High Levels of Endemicity of 3-Chlorobenzoate-Degrading Soil Bacteria}, volume={64}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.64.5.1620-1627.1998}, DOI={10.1128/aem.64.5.1620-1627.1998}, number={5}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Fulthorpe, R. R. and Rhodes, A. N. and Tiedje, J. M.}, year={1998}, month=may, pages={1620–1627} }

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