DOI: 10.1128/aem.64.2.795-799.1998. Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

10.1128/aem.64.2.795-799.1998

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

Bibliography

Marchesi, J. R., Sato, T., Weightman, A. J., Martin, T. A., Fry, J. C., Hiom, S. J., & Wade, W. G. (1998). Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Applied and Environmental Microbiology, 64(2), 795â799.

Authors 7

Julian R. Marchesi (first)
Takuichi Sato (additional)
Andrew J. Weightman (additional)
Tracey A. Martin (additional)
John C. Fry (additional)
Sarah J. Hiom (additional)
William G. Wade (additional)

References 15 Referenced 1,363

10.1128/mr.59.1.143-169.1995
Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. 1989 Greene Publishing Associates-Wiley Interscience New York N.Y
Baranton G. Personal communication.
10.1073/pnas.75.10.4801
10.1128/aem.62.3.872-879.1996
10.1073/pnas.89.12.5685
Lane D. J. 16S/23S rRNA sequencing Nucleic acid techniques in bacterial systematics. Stackebrandt E. Goodfellow M. 1991 115 175 John Wiley & Sons Chichester United Kingdom
Parkes R. J. Cragg B. A. Fry J. C. Herbert R. A. Wimpenny J. W. T. Bacterial biomass and activity in deep sediment layers from the Peru margin. Philos. Trans. R. Soc. Lond. B 133 1990 139 153 / Philos. Trans. R. Soc. Lond. B / Bacterial biomass and activity in deep sediment layers from the Peru margin. by Parkes R. J. (1990)
10.1128/aem.60.6.2113-2119.1994
10.1111/j.1574-6968.1992.tb05682.x
Rochelle P. A. Will J. A. K. Fry J. C. Jenkins G. J. S. Parkes R. J. Turley C. M. Weightman A. J. Extraction and amplification of 16S rRNA genes from deep marine sediments and seawater to assess bacterial community diversity Nucleic acids in the environment: methods and applications. van Elsas J. D. Trevors J. T. 1995 219 239 Springer-Verlag Berlin Germany (10.1007/978-3-642-79050-8_11)
Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y
10.1093/nar/25.1.111
10.1128/aem.62.10.3557-3559.1996
10.1128/mr.51.2.221-271.1987

Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 2:31 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 12:45 a.m.)
Indexed	16 hours, 8 minutes ago (Aug. 30, 2025, 12:27 p.m.)
Issued	27 years, 6 months ago (Feb. 1, 1998)
Published	27 years, 6 months ago (Feb. 1, 1998)
Published Print	27 years, 6 months ago (Feb. 1, 1998)

Funders 0

None

BibTeX

@article{Marchesi_1998, title={Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA}, volume={64}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.64.2.795-799.1998}, DOI={10.1128/aem.64.2.795-799.1998}, number={2}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Marchesi, Julian R. and Sato, Takuichi and Weightman, Andrew J. and Martin, Tracey A. and Fry, John C. and Hiom, Sarah J. and Wade, William G.}, year={1998}, month=feb, pages={795–799} }

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