DOI: 10.1128/aem.64.10.3869-3877.1998. Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation

Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation

10.1128/aem.64.10.3869-3877.1998

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

ABSTRACT A culture-independent molecular phylogenetic approach was used to survey constituents of microbial communities associated with an aquifer contaminated with hydrocarbons (mainly jet fuel) and chlorinated solvents undergoing intrinsic bioremediation. Samples were obtained from three redox zones: methanogenic, methanogenic-sulfate reducing, and iron or sulfate reducing. Small-subunit rRNA genes were amplified directly from aquifer material DNA by PCR with universally conserved or Bacteria - or Archaea -specific primers and were cloned. A total of 812 clones were screened by restriction fragment length polymorphisms (RFLP), approximately 50% of which were unique. All RFLP types that occurred more than once in the libraries, as well as many of the unique types, were sequenced. A total of 104 (94 bacterial and 10 archaeal) sequence types were determined. Of the 94 bacterial sequence types, 10 have no phylogenetic association with known taxonomic divisions and are phylogenetically grouped in six novel division level groups (candidate divisions WS1 to WS6); 21 belong to four recently described candidate divisions with no cultivated representatives (OP5, OP8, OP10, and OP11); and 63 are phylogenetically associated with 10 well-recognized divisions. The physiology of two particularly abundant sequence types obtained from the methanogenic zone could be inferred from their phylogenetic association with groups of microorganisms with a consistent phenotype. One of these sequence types is associated with the genus Syntrophus ; Syntrophus spp. produce energy from the anaerobic oxidation of organic acids, with the production of acetate and hydrogen. The organism represented by the other sequence type is closely related to Methanosaeta spp., which are known to be capable of energy generation only through aceticlastic methanogenesis. We hypothesize, therefore, that the terminal step of hydrocarbon degradation in the methanogenic zone of the aquifer is aceticlastic methanogenesis and that the microorganisms represented by these two sequence types occur in syntrophic association.

Bibliography

Dojka, M. A., Hugenholtz, P., Haack, S. K., & Pace, N. R. (1998). Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation. Applied and Environmental Microbiology, 64(10), 3869â3877.

Authors 4

Michael A. Dojka (first)
Philip Hugenholtz (additional)
Sheridan K. Haack (additional)
Norman R. Pace (additional)

References 48 Referenced 595

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10.1111/j.1745-6592.1995.tb00522.x
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Borden R. C. Natural bioremediation of hydrocarbon-contaminated ground water Handbook of bioremediation. Norris R. D. 1994 177 199 CRC Press Inc. Boca Raton Fla
10.1128/aem.62.6.1935-1943.1996
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10.1111/j.1574-6968.1989.tb03382.x
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10.1128/aem.62.4.1171-1177.1996
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Haack S. K. P. Adriaens F. H. Chapelle L. J. Forney M. A. Henry and D. T. Long. 1998. Unpublished data.
Huffman G. C. Cummings T. R. Gillespie J. L. Brannen J. R. Wurtsmith Air Force Base Michigan. Investigation of soil and ground water contamination at selected sites. Installation Restoration Program Phase II—Confirmation/Quantification Stage 2. U.S. 1995 Geological Survey Lansing Mich
10.1128/JB.180.18.4765-4774.1998
Hugenholtz P. Hershberger K. L. Flanagan J. L. Kimmel B. Pace N. R. Widespread distribution of a novel phylum-depth bacterial lineage in nature abstr. N-23 Abstracts of the 97th General Meeting of the American Society for Microbiology 1997. 1997 385 American Society for Microbiology Washington D.C
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10.1016/0304-3894(96)01753-0
10.1128/aem.63.9.3614-3621.1997
Lane D. J. 16S/23S rRNA sequencing Nucleic acid techniques in bacterial systematics. Stackebrandt E. Goodfellow M. 1991 115 175 John Wiley & Sons Inc. New York N.Y
10.1073/pnas.82.20.6955
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Nozhevnikova A. N. Kotsyurbenko O. R. Simankova M. V. Acetogenesis at low temperature Acetogenesis. Drake H. L. 1994 416 431 Chapman & Hall Ltd. London England (10.1007/978-1-4615-1777-1_15)
10.1128/aem.62.2.461-468.1996
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Pace N. R. Stahl D. A. Lane D. J. Olsen G. J. Analyzing natural microbial populations by rRNA sequences. ASM News 51 1985 4 12 / ASM News / Analyzing natural microbial populations by rRNA sequences. by Pace N. R. (1985)
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10.1016/S0723-2020(11)80449-2
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Dates

Type	When
Created	5 years, 8 months ago (Dec. 19, 2019, 1:54 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 12:37 a.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9:26 a.m.)
Issued	26 years, 11 months ago (Oct. 1, 1998)
Published	26 years, 11 months ago (Oct. 1, 1998)
Published Print	26 years, 11 months ago (Oct. 1, 1998)

Funders 0

None

BibTeX

@article{Dojka_1998, title={Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation}, volume={64}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.64.10.3869-3877.1998}, DOI={10.1128/aem.64.10.3869-3877.1998}, number={10}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Dojka, Michael A. and Hugenholtz, Philip and Haack, Sheridan K. and Pace, Norman R.}, year={1998}, month=oct, pages={3869–3877} }

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