DOI: 10.1128/aem.56.6.1919-1925.1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

10.1128/aem.56.6.1919-1925.1990

Crossref journal-article

American Society for Microbiology

Applied and Environmental Microbiology (235)

Abstract

Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary to short sequence elements within the 16S rRNA common to phylogenetically coherent assemblages of microorganisms, were labeled with tetramethylrhodamine and hybridized to suspensions of fixed cells. Flow cytometry was used to resolve individual target and nontarget bacteria (1 to 5 microns) via probe-conferred fluorescence. Target cells were quantified in an excess of nontarget cells. The intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.

Bibliography

Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., & Stahl, D. A. (1990). Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Applied and Environmental Microbiology, 56(6), 1919â1925.

Authors 6

R I Amann (first)
B J Binder (additional)
R J Olson (additional)
S W Chisholm (additional)
R Devereux (additional)
D A Stahl (additional)

References 0 Referenced 3,043

None

Dates

Type	When
Created	5 years, 8 months ago (Dec. 31, 2019, 11:25 p.m.)
Deposited	3 years, 6 months ago (Feb. 23, 2022, 3:49 p.m.)
Indexed	1 day, 7 hours ago (Aug. 31, 2025, 7:32 p.m.)
Issued	35 years, 3 months ago (June 1, 1990)
Published	35 years, 3 months ago (June 1, 1990)
Published Print	35 years, 3 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{Amann_1990, title={Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations}, volume={56}, ISSN={1098-5336}, url={http://dx.doi.org/10.1128/aem.56.6.1919-1925.1990}, DOI={10.1128/aem.56.6.1919-1925.1990}, number={6}, journal={Applied and Environmental Microbiology}, publisher={American Society for Microbiology}, author={Amann, R I and Binder, B J and Olson, R J and Chisholm, S W and Devereux, R and Stahl, D A}, year={1990}, month=jun, pages={1919–1925} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        31
      ]
    ],
    "date-time": "2025-08-31T23:32:34Z",
    "timestamp": 1756683154383
  },
  "reference-count": 0,
  "publisher": "American Society for Microbiology",
  "issue": "6",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1990,
            6,
            1
          ]
        ],
        "date-time": "1990-06-01T00:00:00Z",
        "timestamp": 644198400000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        6
      ]
    ]
  },
  "abstract": "<jats:p>Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary to short sequence elements within the 16S rRNA common to phylogenetically coherent assemblages of microorganisms, were labeled with tetramethylrhodamine and hybridized to suspensions of fixed cells. Flow cytometry was used to resolve individual target and nontarget bacteria (1 to 5 microns) via probe-conferred fluorescence. Target cells were quantified in an excess of nontarget cells. The intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.</jats:p>",
  "DOI": "10.1128/aem.56.6.1919-1925.1990",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2020,
        1,
        1
      ]
    ],
    "date-time": "2020-01-01T04:25:21Z",
    "timestamp": 1577852721000
  },
  "page": "1919-1925",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 3043,
  "title": "Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations",
  "prefix": "10.1128",
  "volume": "56",
  "author": [
    {
      "given": "R I",
      "family": "Amann",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    },
    {
      "given": "B J",
      "family": "Binder",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    },
    {
      "given": "R J",
      "family": "Olson",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    },
    {
      "given": "S W",
      "family": "Chisholm",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    },
    {
      "given": "R",
      "family": "Devereux",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    },
    {
      "given": "D A",
      "family": "Stahl",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Veterinary Pathobiology, University of Illinois, Urbana 61801."
        }
      ]
    }
  ],
  "member": "235",
  "container-title": "Applied and Environmental Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/aem.56.6.1919-1925.1990",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/aem.56.6.1919-1925.1990",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        2,
        23
      ]
    ],
    "date-time": "2022-02-23T20:49:53Z",
    "timestamp": 1645649393000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/aem.56.6.1919-1925.1990"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        6
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1990,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/aem.56.6.1919-1925.1990"
  ],
  "URL": "http://dx.doi.org/10.1128/AEM.56.6.1919-1925.1990",
  "relation": {},
  "ISSN": [
    "0099-2240",
    "1098-5336"
  ],
  "subject": [],
  "container-title-short": "Appl Environ Microbiol",
  "published": {
    "date-parts": [
      [
        1990,
        6
      ]
    ]
  },
  "assertion": [
    {
      "value": "1990-06-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}